耐盐菌Halomonas alimentaria NY处理高盐废水的研究  

Research on the Treatment of High-salt Wastewater by the Salt-tolerant Bacterium Halomonas Alimentaria NY

作　　者：孟欣 张磊 丁闻 田凤蓉 王开春 王淑军 MENG Xin;ZHANG Lei;DING Wen;TIAN Feng-rong;WANG Kai-chun;WANG Shu-jun(Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology,Jiangsu Ocean University,Lianyungang 222000,China;China BlueStar Lehigh Engineering Corp.,Lianyungang 222004,China)

机构地区：[1]江苏海洋大学海洋生物资源与环境重点实验室/海洋生物技术重点实验室,江苏连云港222000 [2]中蓝连海设计研究院有限公司,江苏连云港222004

出　　处：《化工管理》2023年第23期61-64,86,共5页Chemical Engineering Management

基　　金：江苏海洋大学青年基金,用于废水处理的高产四氢嘧啶嗜盐菌的筛选及应用研究(KQ20015);江苏省高等教育优势学科(PAPD)。

摘　　要：石油、医药和海水养殖等产业的废水具有高盐高氮的特点。采用物理化学法处理存在成本高和效率低的弊端,生物法具有成本低和无二次污染等优点。文章从连云港赣榆盐场沉积土中筛选出耐盐菌株NY,经过16S rDNA基因序列鉴定菌株NY为盐单胞菌(Halomonas alimentaria)。该菌生长的最适碳氮源分别为麦芽糖和酵母粉,最适生长温度和盐度分别为30℃和4%。在初始氮浓度为100~250 mg/L时,该菌能够在24 h内将硝酸盐(NO_(3)^(-))转化为亚硝酸盐(NO_(2)^(-)),硝酸盐的降解率均大于80%。该菌在高盐含氮废水的处理中具有较好的应用前景。Wastewater from petroleum,medicine and mariculture industries is characterized by high salinity and high nitrogen.The physical-chemical method has the disadvantages of high cost and low eff iciency,while the biological method has the advantages of low cost and no secondary pollution.A salt-tolerant strain NY was screened from the sedimentary soil of Ganyu Salt Farm in Lianyungang.The 16S rDNA gene sequence was identif ied the strain NY as Halomonas alimentaria.The bacteria could grow normally under the salinity of 20%,the optimum growth initial pH was 6.0,and the optimum carbon and nitrogen sources were maltose and yeast powder.When the initial nitrogen concentration was 100-250 mg/L,the bacteria could convert nitrate(NO_(3)^(-))to nitrite(NO_(2)^(-))within 24 h,and the nitrate degradation rate was more than 80%.This bacterium has a great application prospect in the treatment of high-salt and nitrogen-containing wastewater.

关 键 词：高盐废水 耐盐菌 反硝化 高效转化 

分 类 号：X703[环境科学与工程—环境工程]