4例青春期前男孩睾丸组织冷冻保存手术护理配合  

Nursing cooperation in surgical collection of testis tissues from prepubertal male patients for cryopreservation:A study of 4 cases

作　　者：孙德娟[1] 郑雅露 潘慧玲 高潇 陈红 康晓梅 李俐琳[1] SUN De-juan;ZHENG Ya-lu;PAN Hui-ling;GAO Xiao;CHEN Hong;KANG Xiao-mei;LI Li-lin(Center of Reproductive Medicine,The Sixth Hospital Affiliated to Sun Yat-sen University,Guangzhou,Guangdong 510655,China)

机构地区：[1]中山大学附属第六医院生殖医学中心,广东广州510655

出　　处：《中华男科学杂志》2023年第1期60-65,共6页National Journal of Andrology

摘　　要：目的:探讨青春期前男孩睾丸组织冷冻保存手术护理配合的方法。方法:回顾性分析生育力保存的4例青春期前男孩睾丸组织冷冻保存围手术期护理方法与效果,重点阐述与睾丸组织保护相关的术前、术中无菌冰盅的制作准备及使用注意事项,术中睾丸组织离体后的保护以及转运等细节。结果:进行生育力保存的4例青春期前男孩手术均顺利完成,快速恢复,获取的睾丸组织得到良好保护并顺利转运到胚胎实验室,组织状态好,规范处理后采用慢速程序化冷冻方法分别为4例患者冷冻了31、31、20、34块睾丸组织,为患者未来恢复、维持男性内分泌功能、生育自己的后代提供了可能。结论:对于需进行生育力保存的男孩,睾丸组织获取术围手术期无菌冰盅的准备、使用及术中护理操作配合、组织保护转运等措施,可以实现睾丸组织的有效保护,为后续胚胎实验室睾丸组织的处理及冷冻奠定了基础,取得了很好的护理效果,也为生育力保存护理的推进提供借鉴和参考。Objective:To explore nursing cooperation in surgical collection of the testis tissue from prepubertal male patients for cryopreservation.Methods:We retrospectively analyzed the methods and effects of perioperative nursing in surgical collection of the testis tissue from 4 prepubertal male patients for cryopreservation in our Center of Reproductive Medicine.Before,during and after operation,we took strict measures in making sterilized ice containers,intraoperative nursing cooperation,protection of the isolated testis tissues and transferring of the samples.Results:Testis tissues were successfully collected from all the 4 prepubertal males,31,31,20 and 34 samples from each case respectively,well protected and subjected to slow cryopreservation after standard processing in the embryo laboratory.Conclusion:In surgical collection of the testis tissue for cryopreservation,preparation of sterilized ice containers,intraoperative nursing cooperation and protection and transferring of the samples are essential for standard processing and cryopreservation of the testis tissue in the embryo laboratory.

关 键 词：青春期前男孩 睾丸组织冷冻 护理配合 生育力保存 

分 类 号：R473.6[医药卫生—护理学] R699.8[医药卫生—临床医学]