柿属植物组织培养技术研究进展  

Research Progress on Tissue Culture of Diospyros

作　　者：吕中一 关长飞 李家艳 丁瑜 范芝蕊 杨勇[1] LYU Zhongyi;GUAN Changfei;LI Jiayan;DING Yu;FAN Zhirui(College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100)

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《北方园艺》2023年第12期129-137,共9页Northern Horticulture

基　　金：陕西省创新能力支撑计划资助项目(2022PT-25);陕西省重点研发计划资助项目(2022NY-122)。

摘　　要：柿属(Diospyros)植物组织培养技术的发展应用可大幅度缩短良种苗木培育周期,为柿属植物育种提供高效新途径,且为遗传转化体系建立及基因功能研究提供技术支撑。该文综述了国内外柿属植物组织培养技术的研究进展,着重对不同柿品种、外植体类型、消毒方法、植物激素类型和配比、基本培养基等因素对柿组组织培养的影响进行系统性阐述,并对柿属植物组织培养过程出现的重点难题进行探讨,全面概括分析外植体材料易褐化死亡、组培苗生根困难等问题,以期为国内柿属植物高效组培体系的建立提供参考依据。The development and application of tissue culture technology of Diospyros can greatly shorten the cultivation cycle of good seedlings,provide an efficient new way for Diospyros breeding,and provide technical support for the establishment of genetic transformation system and gene function research.This study summarized the progress of Diospyros tissue culture technology at home and abroad,focusing on different persimmon varieties,implant type,disinfection methods,plant hormone type and ratio,basic medium and other factors on the influence of persimmon tissue culture,and discussed the key problems,a comprehensive analysis of graft material easy to browning death,tissue culture seedling root difficulties,in order to provide reference for the establishment of domestic persimmon tissue culture system.

关 键 词：柿 组织培养 研究进展 

分 类 号：S665[农业科学—果树学]