黄花菜维生素E含量遗传分析及QTL定位  

Genetic analysis and QTL localization of vitamin E content in Hemerocallis citrina Baroni

作　　者：段春宇 熊雄 景梦岳 高阳 侯非凡 邢国明[1] 李森[1] DUAN Chunyu;XIONG Xiong;JING Mengyue;GAO Yang;HOU Feifan;XING Guoming;LI Sen(College of Horticulture,Shanxi Agricultural university/Datong Daylily Industrial Development Research Instiute,Datong 037004,China)

机构地区：[1]山西农业大学园艺学院/大同黄花产业发展研究院,山西太谷030801

出　　处：《河北农业大学学报》2023年第4期38-45,共8页Journal of Hebei Agricultural University

基　　金：2021年山西省研究生创新项目(2021Y323);国家重点研发计划项目子课题(2021YFD1600301-2);山西农业大学生物育种工程项目(YZGC122);2021年度大同黄花产业发展研究院科研合作项目(2022QT003-1).

摘　　要：维生素E是黄花菜中重要的营养成分。本研究以黄花菜地方品种‘东庄黄花’和‘冲里花’杂交获得的F1群体为研究对象,对杂交后代连续2年的维生素E含量进行检测分析,结果显示黄花菜F1群体维生素E含量的变异系数为50.44%~54.23%,变异系数较大,整体呈现正态分布趋势,并出现了含量超过双亲的超亲后代个体;遗传分析显示黄花菜维生素E含量符合2对主基因-加性-显性模型(2MG-AD),第1对基因加性效应更为显著,主基因遗传效率高达95%以上;对黄花菜维生素E含量进行QTL定位,共检测到2个与黄花菜维生素E相关的QTL,分布于8号和10号连锁群上,LOD峰值的范围为2.96~3.60,单个QTL贡献率介于10.80%~13.00%。本研究结果为黄花菜高维生素E含量的新品种选育和品种改良提供了数据基础。Vitamin E is an important nutrient in Hemerocallis citrina Baroni.In this study,the F1 population obtained by crossing the local variety of Hemerocallis citrina Baroni‘Dongzhuang Huanghua’and‘Chonglihua’was used as the research object,and the vitamin E content of the cross progeny was examined and analyzed for 2 consecutive years.The results showed that the vitamin E content in the F1 pulation of Hemerocallis citrina Baroni displayed an normal distribution with a large coefficient of variation that ranged from 50.44%to 54.23%.There were super-parental progeny whose content exceeded that of both parents.Genetic analysis showed that the vitamin E content of Hemerocallis citrina conformed to the additive-dominance model controlled by two major genes(2MG-AD).The genetic efficiency of the main genes are over 95%.QTL analysis has identified two locus related to vitamin E in Hemerocallis citrina Baroni.The two locus distributed on the linkage group 8 and 10,and the range of LOD peak was 2.96-3.60. The contribution rate of individual QTL ranged from 10.80% to 13.00%. The results of this study provide a data base for the selection of new varieties and variety improvement of Hemerocallis citrina Baroni with high vitamin E content.

关 键 词：黄花菜 维生素E 遗传分析 QTL定位 

分 类 号：S644.3[农业科学—蔬菜学]