6-OHDA诱导的PD模型小鼠的运动及焦虑症状  

MOTOR AND ANXIETY SYMPTOMS IN A MOUSE MODEL OF 6-HYDROXYDOPAMINE-INDUCED PARKINSON’S DISEASE

作　　者：陈凤华 石丽敏[1] 谢俊霞[1] CHEN Fenghua;SHI Limin;XIE Junxia(Department of Physiology and Pathophysiology,School of Basic Medicine,Qing-dao University,Institute of Brain Sciences and Diseases,Qingdao 266071,China)

机构地区：[1]青岛大学基础医学院生理学与病理生理学系,脑科学与疾病研究院,山东青岛266071

出　　处：《青岛大学学报（医学版）》2023年第3期321-324,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金面上项目(32171132)。

摘　　要：目的探讨6-羟基多巴胺(6-OHDA)诱导的单侧帕金森病(PD)模型小鼠的运动及焦虑症状。方法7周龄雄性C57BL/6小鼠20只,随机分为对照组及模型组,每组10只。模型组小鼠通过左侧纹状体立体定位注入6-OHDA(2 g/L,2μL)制备PD模型,对照组小鼠注入等量的生理盐水。2周后进行旷场实验检测小鼠的移动总距离和中心区探索时间,采用酪氨酸羟化酶(TH)免疫荧光染色检测黑质区多巴胺能神经元数目。结果旷场实验结果显示,与对照组相比,模型组小鼠移动总距离明显减少,中心区探索时间明显增加,差异具有统计学意义(t=2.201、2.576,P<0.01)。免疫荧光染色结果显示,与对照组相比,模型组小鼠黑质区TH阳性神经元的数目明显减少,差异有统计学意义(t=17.570,P<0.001)。结论6-OHDA诱导的单侧PD模型小鼠黑质-纹状体系统功能受损,出现运动障碍但没有产生焦虑。Objective To investigate the motor and anxiety symptoms in a mouse model of 6-hydroxydopamine(6-OHDA)-induced unilateral Parkinson’s disease(PD).Methods A total of 20 male C57BL/6 mice,aged 7 weeks,were randomly divided into control group and model group,with 10 mice in each group.The mice in the model group were given stereotactic injection of 2μL 6-OHDA(2 g/L)into the left corpus striatum to establish a model of PD,and those in the control group were gi-ven injection of an equal volume of normal saline.Two weeks later,the open field test was used to measure total moving distance and time spent in the center of the open field,and tyrosine hydroxylase(TH)immunofluorescent staining was used to measure the number of dopaminergic neurons in the substantia nigra.Results The open field test showed that compared with the control group,the model group had a significant reduction in total moving distance and a significant increase in time spent in the center of the open field(t=2.201,2.576;P<0.01).Immunofluorescent staining showed that compared with the control group,the model group had a significant reduction in the number of TH-positive neurons in the substantia nigra(t=17.570,P<0.001).Conclusion Impaired function of the substantia nigra-corpus striatum system is observed in a mouse model of 6-OHDA-induced unilateral PD,with the presence of movement disorders,but without the presence of anxiety.

关 键 词：帕金森病 羟多巴胺 纹状体 小鼠 症状评估 

分 类 号：R338.2[医药卫生—人体生理学]