Kv7钾离子通道对大鼠条件性味觉厌恶记忆作用  

EFFECT OF KV7 CHANNEL ON CONDITIONED TASTE AVERSION MEMORY IN RATS

作　　者：高珊珊[1] 杨鑫 付嘉豪 邵非 翟洪霞 周宇[1] GAO Shanshan;YANG Xin;FU Jiahao;SHAO Fei;ZHAI Hongxia;ZHOU Yu(Department of Physiology and Pathophysiology,School of Basic Medicine,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学基础医学院生理学教研室,山东青岛266071

出　　处：《青岛大学学报（医学版）》2023年第3期364-366,共3页Journal of Qingdao University(Medical Sciences)

基　　金：山东省自然科学基金青年基金项目(ZR201911-120651)。

摘　　要：目的探讨基底外侧杏仁核(BLA)微量注射Kv7钾离子通道激动剂或拮抗剂对大鼠条件性味觉厌恶(CTA)情绪记忆获取及提取的影响。方法成年雄性Wistar大鼠BLA埋管后随机分为8组,实验组分别于不同时间经BLA微量注射Kv7钾离子通道激动剂(retigabine)或拮抗剂(XE-991),其对照组注射生理盐水。采用CTA行为范式评价Kv7通道激动剂及拮抗剂对CTA情绪记忆获取及提取的影响。结果训练前注射retigabine组大鼠味觉厌恶指数较注射生理盐水组大鼠明显下降,差异有统计学意义(t=3.384,P<0.05);记忆提取前注射retigabine组大鼠味觉厌恶指数与注射生理盐水组大鼠比较差异无显著性(P>0.05)。训练前和记忆提取前注射XE-991组大鼠与注射生理盐水组大鼠相比,味觉厌恶指数差异均无显著性(P>0.05)。结论Kv7钾离子通道的激活抑制CTA记忆获取,该作用可能与降低BLA神经元兴奋性有关。Objective To investigate the effect of microinjection of Kv7 channel agonist or antagonist into the basolateral amygdala(BLA)on the acquisition and retrieval of conditioned taste aversion(CTA)memory in rats.Methods Adult male Wistar rats were randomly divided into eight groups after BLA catheter embedding.The rats in experimental groups were given intra-BLA microinjection of Kv7 channel agonist(retigabine)or antagonist(XE-991),and those in control groups were given injection of normal saline.The CTA behavioral paradigm was used to evaluate the effect of Kv7 channel agonist or antagonist on the acquisition and retrieval of memory.Results Compared with the rats injected with normal saline,the rats injected with retigabine before CTA training had a significant reduction in taste aversion index(t=3.384,P<0.05),and there was no significant difference in taste aversion index between the rats injected with retigabine and those injected with normal saline before memory retrieval(P>0.05).There was no significant difference in taste aversion index between the rats injected with XE-991 and those injected with normal saline before training and memory retrieval(P>0.05).Conclusion Kv7 channel activation inhibits the acquisition of CTA memory in rats,possibly by reducing BLA neuronal excitability.

关 键 词：钾通道 电压门控 基底外侧核 情绪 记忆 大鼠 

分 类 号：R338.2[医药卫生—人体生理学]