基底外侧杏仁核微量注射ghrelin对大鼠条件性味觉厌恶记忆的影响  

EFFECT OF MICROINJECTION OF GHRELIN IN THE BASOLATERAL AMYGDALA ON CONDITIONED TASTE AVERSION MEMORY IN RATS

作　　者：田欣 裴彤[1] 赵鸿飞 谭雯璐 刘盼盼 周宇[1] TIAN Xin;PEI Tong;ZHAO Hongfei;TAN Wenlu;LIU Panpan;ZHOU Yu(State Key Discipline:Physioloy(in Incubation),Department of Physiology,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学国家生理学重点(培育)学科,山东青岛266071

出　　处：《青岛大学学报（医学版）》2023年第3期367-370,共4页Journal of Qingdao University(Medical Sciences)

基　　金：中国博士后科学基金面上资助项目(2019M6622-92)。

摘　　要：目的探讨胃促生长素(ghrelin)对条件性味觉厌恶(CTA)记忆的影响及其可能的分子机制。方法将成年SD大鼠随机分为home cage组(未处理组)、对照组和实验组,CTA训练前20 min实验组大鼠双侧基底外侧杏仁核(BLA)注射15μmol/L的ghrelin每侧0.5μL,对照组大鼠BLA注射等量生理盐水。CTA训练24 h后进行记忆测试,用厌恶指数(AI)对CTA记忆进行量化分析。CTA实验结束30 min后取BLA组织,采用实时荧光定量PCR方法检测电压门控钾通道1(Kcna1)和电压门控钾通道4(Kcna4)的转录水平。结果实验组AI明显低于对照组,差异具有统计学意义(t=3.052,P<0.05)。与home cage组相比,CTA训练后对照组大鼠Kcna1转录水平下调(F=3.743,P<0.05),Kcna4转录水平上调(F=18.300,P<0.001);而实验组大鼠Kcna1和Kcna4的表达与home cage组比较差异均无显著性(P>0.05)。结论CTA训练引起BLA脑区Kcna1和Kcna4表达的可塑性变化。BLA微量注射ghrelin抑制大鼠CTA记忆的获取,这可能与ghrelin抑制BLA脑区Kcna1和Kcna4表达的可塑性变化有关。Objective To investigate the effect of ghrelin on conditioned taste aversion(CTA)memory and to explore the possible molecular mechanism.Methods Adult Sprague Dawley rats were randomly divided into home cage group(untreated group),control group,and experimental group.Rats in the experimental group received a single dose of ghrelin(15μmol/L,0.5μL each side)in the bilateral basolateral amygdala(BLA)20 min before CTA training,and rats in the control group were administrated an equal volume of normal saline in the BLA.Memory was evaluated 24 h after CTA training and quantitatively analyzed by the aversive index(AI).BLA tissues were obtained 30 min after CTA training,and quantitative real-time polymerase chain reaction was done to measure the mRNA expression of voltage-gated potassium channel 1(Kcna1)and voltage-gated potas-sium channel 4(Kcna4).Results The AI in the experimental group was significantly lower than that in the control group(t=3.052,P<0.05).Compared with the home cage group,the control group had significantly downregulated Kcna1 transcript(F=3.743,P<0.05)but significantly upregulated Kcna4 transcript(F=18.300,P<0.001)after CTA training.However,the expression of both Kcna1 and Kcna4 transcripts showed no significant differences between the experimental group and the home cage group(P>0.05).Conclusion CTA training triggers plasticity changes in Kcna1 and Kcna4 expression in the BLA brain region.Ghrelin microinjected into the BLA inhibits CTA memory acquisition in rats,which may be associated with the suppressive effect of ghrelin on plasticity changes in Kcna1 and Kcna4 expression in the BLA brain region.

关 键 词：胃促生长素 基底外侧核 钾通道 电压门控 记忆 大鼠 

分 类 号：R338.2[医药卫生—人体生理学]