LB对IL-17A诱导HaCaT细胞增殖和细胞因子影响  

EFFECT OF LOUREIRIN B ON THE PROLIFERATION AND CYTOKINE SECRETION OF HACAT CELLS INDUCED BY IL-17A

作　　者：冯世军[1] 马敬[1] 李华蕊[2] 刘远 艾东方[1] 楚瑞琦[3] FENG Shijun;MA Jing;LI Huarui;LIU Yuan;AI Dongfang;CHU Ruiqi(Department of Dermatology,The Central Hospital of Cangzhou,Cangzhou 061001,China)

机构地区：[1]沧州市中心医院皮肤性病科,河北沧州061001 [2]沧州市中心医院营养科 [3]河北大学附属医院皮肤性病科

出　　处：《青岛大学学报（医学版）》2023年第3期416-419,共4页Journal of Qingdao University(Medical Sciences)

基　　金：河北省医学科学研究课题计划项目(20190188)。

摘　　要：目的探讨龙血素B(LB)对白细胞介素-17(IL-17A)诱导的HaCaT细胞增殖和细胞因子分泌的影响及其机制。方法采用噻唑蓝检测细胞增殖;酶联免疫吸附检测IL-6和IL-23表达,实时荧光定量PCR(qRT-PCR)检测趋化因子(CXCL1、CXCL12、CXCL20)mRNA表达量,蛋白免疫印迹方法检测Janus激酶-信号转导子及转录激活子(JAK-STAT)通路相关蛋白Janus激酶1(JAK1)、磷酸化信号转导子和转录激活子3(p-STAT3)、信号转导子和转录激活子3(STAT3)的表达。结果与对照组相比,IL-17A组的吸光度值(A值)及IL-6、IL-23和CXCL1、CXCL12和CXCL20 mRNA表达明显增加(F=34.76~135.70,P<0.001),JAK1、p-STAT3和STAT3蛋白表达上调(F=53.91~105.70,P<0.001)。IL-17A可以剂量依赖性地降低HaCaT细胞的A值,IL-6、IL-23,CXCL1、CXCL12、CXCL20 mRNA含量及JAK1、p-STAT3、STAT3蛋白量(F=22.55~88.41,P<0.001)。与IL-17A+LB-H组相比,IL-17A+LB-H+AG490组的A值、IL-6、IL-23和CXCL1、CXCL12、CXCL20 mRNA明显降低(t=4.73~8.40,P<0.001)。结论LB能抑制IL-17A诱导的HaCaT细胞增殖及相关细胞因子分泌,其作用机制可能与抑制JAK-STAT通路有关。Objective To investigate the effect of loureirin B(LB)on the proliferation and cytokine secretion of HaCaT cells induced by interleukin-17A(IL-17A).Methods MTT assay was used to determine cell proliferation;enzyme-linked immunosorbent assay was used to measure the expression of interleukin-6(IL-6)and interleukin-23(IL-23);quantitative real-time polymerase chain reaction was used to measure the mRNA expression of chemokines(CXCL1,CXCL12,CXCL20);Western blotting was used to measure the protein expression of Janus kinase 1(JAK1),phosphorylated signal transducer and activator of transcription 3(p-STAT3),and signal transducer and activator of transcription 3(STAT3).Results Compared with the control group,the IL-17A group showed significant increases in absorbance,the levels of IL-6 and IL-23,and the mRNA expression of CXCL1,CXCL12,and CXCL20(F=34.76-135.70,P<0.001),as well as significant upregulation of the protein expression of JAK1,p-STAT3,and STAT3(F=53.91-105.70,P<0.001).LB reduced the absorbance,IL-6 and IL-23 levels,CXCL1,CXCL12,and CXCL20 mRNAs,and JAK1,p-STAT3,and STAT3 proteins of HaCaT cells in a dose-dependent manner(F=22.55-88.41,P<0.001).Compared with the IL-17A+LB-H group,the IL-17A+LB-H+AG490 group showed significant reductions in absorbance,IL-6 and IL-23 levels,and CXCL1,CXCL12,and CXCL20 mRNAs(t=4.73-8.40,P<0.001).Conclusion LB can inhibit the proliferation and cytokine secretion of HaCaT cells induced by IL-17A,possibly by suppressing the JAK-STAT pathway.

关 键 词：龙血素B HACAT细胞 白细胞介素17 细胞增殖 细胞因子类 JAK-STAT通路 银屑病 

分 类 号：R284[医药卫生—中药学] R73-3[医药卫生—中医学]