槲皮素通过TLR4/MyD88途径改善LPS诱导的bEECs的炎症反应  

Quercetin improved LPS-induced inflammatory response inbovine endometrial epithelial cells through TLR4/MyD88 pathway

作　　者：张雪 石雨欣 李相辰 宋亮丽 ZHANG Xue;SHI Yuxin;LI Xiangchen;SONG Liangli(College of Animal Science and Technology,Ningxia University,Ningxia Yinchuan,750021,China)

机构地区：[1]宁夏大学动物科技学院,银川750021

出　　处：《北京农学院学报》2023年第3期73-76,共4页Journal of Beijing University of Agriculture

基　　金：宁夏自然科学基金资助项目(2021AAC03040);宁夏重点研发(引才专项)资助(2020BEB04035)。

摘　　要：【目的】旨在探明槲皮素对LPS诱导的奶牛子宫内膜上皮细胞(bEECs)炎症反应的作用。【方法】用组织块和差时消化法分离纯化bEECs,1μg/mL脂多糖(LPS)作用12 h后,添加不同浓度槲皮素(10,20,40μM)孵育12 h,采用Western blot法检测TLR4和MyD88蛋白的表达,同时通过qRT-PCR法检测炎症因子IL-1β、IL-6和TNF-αmRNA的相对表达量。【结果】经CK18免疫荧光染色证实获得的bEECs纯度较高。LPS作用后,细胞炎症因子的表达显著高于对照组(P<0.01)。相比于LPS组,槲皮素孵育后,可降低炎症因子和TLR4/MyD88的表达(P<0.01)。【结论】槲皮素可通过抑制TLR4/MyD88的表达,改善LPS诱导的奶牛子宫内膜上皮细胞的炎症反应,该结果将为奶牛子宫内膜炎的治疗提供了新的研究思路和理论依据。[Objective]This experiment was conducted to investigate the effect of quercetin on LPS-induced inflammatory response in bovine endometrial epithelial cells(bEECs).[Methods]The bEECs were isolated and purified by tissue block and differential digestion.After treatment with 1μg/mL lipopolysaccharide(LPS)for 12 h,different concentrations of quercetin(10,20,40μM)were added for incubation for 12 h.Western blot was used to detected the expression of TLR4 and MyD88 proteins.The relative expression of inflammatory factors IL-1β,IL-6 and TNF-αmRNA was detected by qRT-PCR.[Results]The purity of bEECs was confirmed by CK18 immunofluorescence staining.After LPS treatment,the expression of inflammatory factors was significantly higher than that of the control group(P<0.01).Quercetin could reduce the expression of inflammatory factors and TLR4/MyD88 compared with LPS group(P<0.01).[Conclusion]Quercetin can improve the LPS-induced inflammatory response of bovine endometrial epithelial cells by inhibiting the expression of TLR4/MyD88.The results of this study provide new research ideas and theoretical basis for the treatment of bovine endometritis.

关 键 词：子宫内膜炎 奶牛子宫内膜上皮细胞 槲皮素 脂多糖 

分 类 号：S823.91[农业科学—畜牧学]