石榴皮多酚提取物联合二甲双胍对2型糖尿病小鼠模型的治疗作用  

Therapeutic Effect of Pomegranate Peel Polyphenols Extract Combination with Metformin on Type 2 Diabetic Mellitus Mouse Model

作　　者：汪鹏 徐树辉 吴桂冰 李鸿基 李斯锦 黄沛婷 余宗峻 Wang Peng;Xu Shuhui;Wu Guibing(School of Basic Medical Sciences,Guangzhou Medical University,Guangzhou,Guangdong 511436,China)

机构地区：[1]广州医科大学基础医学院,广东广州511436

出　　处：《四川医学》2023年第7期683-688,共6页Sichuan Medical Journal

基　　金：广东省大学生创新创业训练计划项目(编号:S201910570024)。

摘　　要：目的 探究石榴皮多酚(PPP)提取物联合二甲双胍(ME)对2型糖尿病(T2DM)小鼠模型的协同治疗作用。方法 高糖高脂饲料联合链脲佐菌素诱导建立T2DM小鼠模型。连续治疗4周,期间观察并记录小鼠一般状况(GC):进食量(FI)、饮水量(WI)及体质量(BW);小鼠糖耐量相关指标(GTRI):空腹血糖(FBG)、口服糖耐量试验曲线下面积;记录并用空腹胰岛素与空腹血糖计算胰岛素功能相关指标(IFRI):胰岛素抵抗指数、胰岛素敏感性指标和β细胞功能。结果 小鼠在造模成功后的第1周开始出现FI、WI增多,但BW减轻的糖尿病症状。T2DM小鼠经PPP和ME治疗后发现:两药均能显著改善小鼠的WI、BW、GTRI和IFRI,但对FI的影响不明显;两药对小鼠GC、GTRI和IFRI作用的差异无统计学意义。两药联合使用在改善小鼠GTRI和IFRI方面均具有交互作用,且该交互作用在改善除FBG外的其他GTRI和IFRI指标方面均要显著强于单独用药。结论 PPP能有效治疗T2DM,其治疗作用与ME基本相当。当PPP与ME联合使用时,两者存在交互作用以协同治疗T2DM。因此,PPP联合ME有可能成为一种安全、经济、有效的治疗T2DM的新方案,值得进一步研究推广。Objective To explore the synergistic treatment effect of pomegranate peel polyphenol(PPP)extract and metformin(ME)on type 2 diabetes mellitus(T2DM)mouse model.Methods High-sugar and high-fat diet combined with streptozotocin induction was used to establish T2DM mouse model.During 4 weeks of continuous treatment,the general condition of mice(GC):food intake(FI),water intake(WI)and body weight(BW),and glucose tolerance related indicators in mice(GTRI):fasting blood glucose(FBG),oral glucose tolerance test curve-area under the curve were observed and recorded;the fasting insulin and fasting blood glucose were recorded and used to calculate insulin function related indexes(IFRI):insulin resistance index,insulin sensitivity index andβcell function.Results Mice developed diabetic symptoms with increased FI and WI,but reduced BW on the first week after successful modeling.T2DM mice were treated with PPP and ME and were found that:both drugs significantly improved WI,BW,GTRI and IFRI,except for FI.There was no significant difference in the effects of two drugs on mice s GC,GTRI and IFRI.Combined usage of the two drugs had an interactive effect on improving GTRI and IFRI in mice,and the interaction was significantly stronger than monotherapy in improving other GTRI and IFRI indicators except FBG.Conclusion PPP can effectively treat T2DM,and its therapeutic effect is basically the same as that of ME.However,when PPP and ME are used in combination,the two drugs have an interactive effect on the synergistically treatment of T2DM.Therefore,PPP combined with ME may become a safe,economical and effective new treatment plan for T2DM,which is worthy of further research and promotion.

关 键 词：石榴皮多酚 二甲双胍 2型糖尿病 小鼠模型 

分 类 号：R587.1[医药卫生—内分泌]