超声辅助酶提取技术对驼皮源胶原蛋白降解及其活性肽的影响  

Effect of ultrasound-assisted enzyme extraction on the protein degradationand biological of peptides in camel skin collagen

作　　者：何静[1] 史睿 吉日木图[1,2] HE Jing;SHI Rui;Jirimutu(College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China;China-Mongolia Joint Laboratory of Biopolymer Application“One Belt One Road”,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018 [2]中国-蒙古国生物高分子应用“一带一路”联合实验室,内蒙古呼和浩特010018

出　　处：《食品与发酵工业》2023年第15期153-159,共7页Food and Fermentation Industries

基　　金：内蒙古农业大学高层次人才引进科研启动项目(NDYB2019-26);国家重点研发计划项目(2020YFE0203300)。

摘　　要：该研究基于模拟胃肠道消化,采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、粒径分布、液相色谱-串联质谱表征超声辅助酶法提取和传统酶法提取获得的胶原蛋白水解特征。进一步结合分子对接虚拟筛选具有结合潜力的活性肽并探讨其与靶蛋白的结合位点及作用方式。结果表明,超声辅助酶提取技术能促进驼皮胶原蛋白的降解,其肽段的分子质量集中在500~1500 Da。经过虚拟筛选技术确定超声辅助酶法提取技术能够产生更多潜在的活性肽段,以血管紧张素转化酶(angiotensin converting enzyme,ACE)抑制为主。基于分子对接技术获得可能最具潜力的ACE抑制肽LSFLF,该肽通过与ACE活性部位的关键氨基酸残基形成分子间相互作用而产生抑制作用。该研究结果可为驼皮深加工与活性肽的鉴定和制备提供新思路。In this study,the hydrolysis characteristics of collagen obtained by ultrasonic assisted enzymatic extraction and traditional enzymatic extraction were characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis,particle size distribution,and liquid chromatography tandem mass spectrometry based on simulated gastrointestinal digestion.Furthermore,molecular docking was carried out to screen the most potential bioactive peptides with high binding capacity and investigate the binding site and binding mechanism with target protein.The results showed that ultrasonic assisted enzyme extraction promoted the degradation of camel skin collagen,and its molecular mass of peptide fragments was distributed in the range of 500-1500 Da.Through the virtual screening technology,it was confirmed that the ultrasonic-assisted enzymatic extraction could produce more potential bioactive peptides,mainly ACE inhibition.The most potential ACE inhibitory peptide LSFLF was obtained based on molecular docking.It could interact with the key amino acid residues with the active site of ACE.The results provide a new idea for further processing of camel skin and identification and preparation of bioactive peptides.

关 键 词：超声辅助酶法 驼皮胶原蛋白 胃肠道消化 虚拟筛选 

分 类 号：TS251.92[轻工技术与工程—农产品加工及贮藏工程]