短链伯醇氧化酶的研究进展  

Research progress of short-chain primary alcohol oxidase

作　　者：王首丰 丛文杰 曹昕莼 陆婷 王明轩 周化岚[1] 张建国[1] WANG Shoufeng;CONG Wenjie;CAO Xinchun;LU Ting;WANG Mingxuan;ZHOU Hualan;ZHANG Jianguo(School of Health Science and Enginering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学健康科学与工程学院,上海200093

出　　处：《食品与发酵工业》2023年第15期296-304,共9页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31870045)。

摘　　要：真核甲基营养菌中的短链伯醇氧化酶(short-chain primary alcohol oxidase,SPAOX,EC 1.1.3.13)是一种依赖于黄素腺嘌呤二核苷酸(flavin adenine dinucleotide,FAD),能氧化短链伯醇生成相应醛的蛋白质。SPAOX位于真核生物的过氧化物酶体中,是参与醇类代谢的重要酶。SPAOX的催化反应能循环使用FAD,且底物广泛,广泛应用于检测和生物转化生产。SPAOX的聚体结构,使得其在反应和储存过程中容易受到环境和化合物的影响,这限制了其应用。文章从SPAOX的来源、催化机制和结构、底物、稳定性等方面简述了SPAOX的基本特点,为SPAOX的应用和发展提供了基础,也从分子说展望了SPAOX的研究进展,为SPAOX的研究提供参考。Short-chain primary alcohol oxidase from eukaryotic methylotroph is a flavin adenine dinucleotide(FAD)-dependent enzyme that oxidizes short-chain primary alcohols to their corresponding aldehydes.SPAOX locates in peroxisomes of eukaryotes,which is a key enzyme involved in alcohol metabolism.SPAOX has been widely used in detection and biotransformation production due to its wide range of substrate and the recycle of FAD during the reaction.The multimer structure of SPAOX makes it vulnerable to environment and chemicals during the progress of reaction and storage,which limits its application.This review outlines the fundamental characteristics of SPAOX from the perspectives of source,catalytic mechanism and structure,substrate,and stability,puts a foundation for application and development,provides an outlook for future research based on molecular level,and built base for research related to SPAOX.

关 键 词：短链伯醇氧化酶 黄素腺嘌呤二核苷酸 底物 稳定性 

分 类 号：Q55[生物学—生物化学] TQ426.97[化学工程]