三味板蓝根颗粒浸膏制备工艺改良研究  

Preparation Process Improvement of Sanwei Banlan Banlan’gen Granules Extract

作　　者：陈斯宁[1] 陈舒茵[1] 梁国成[1,2,3] 杨红梅 覃翔[1,3] 韦世民[1,3] CHEN Sining;CHEN Shuyin;LIANG Guocheng;YANG Hongmei;QIN Xiang;WEI Shimin(Department of Respiratory and Critical Care/GCP Office/Drug Research and Development Center,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Guangxi Province,Nanning 530011,China;School of Chemistry and Chemical Engineering,Guangxi University,Guangxi Province,Nanning 530004,China;Guangxi Zhuang and Yao Ethnic Medicine Engineering and Technology Research Center,Guangxi Province,Nanning 530200,China)

机构地区：[1]广西中医药大学附属瑞康医院呼吸与重症科/GCP办/药物研发中心,广西南宁530011 [2]广西大学化学化工学院,广西南宁530004 [3]广西壮瑶药工程技术研究中心,广西南宁530200

出　　处：《中国中医药现代远程教育》2023年第16期145-149,共5页Chinese Medicine Modern Distance Education of China

基　　金：广西壮族自治区中医药管理局中药壮瑶药医院制剂质量提升项目【No.GZZJ202010】;广西中医药大学自然科学研究项目【No.2019MS036】。

摘　　要：目的改良医院制剂三味板蓝根颗粒浸膏中间体的制备工艺。方法采用L_(9)(3^(4))正交试验优选浸膏提取工艺的关键参数,再以单因素和Box-Behnken设计-响应面法优化浸膏醇沉工艺。结果正交试验优选的浸膏最佳提取工艺为:以10倍量水(初次浸泡0.5 h),沸腾提取3次,每次提取1.5 h。经单因素和响应面法优化浸膏最佳醇沉工艺为:将药液浓缩至相对密度为1.15(80℃),以95%乙醇将药液的终点醇浓度调至65%,醇沉静置时间48 h。结论采用L_(9)(3^(4))正交试验联合Box-Behnken设计-响应面法优化医院制剂三味板蓝根颗粒的提取和醇沉工艺,达到预期效果,可为实际扩大化生产提供参考。Objective To improve the preparation process of hospital preparation Sanwei Banlan’gen granules extract intermediates.Methods The key parameters of the extract extraction process were optimized by L_(9)(3^(4))orthogonal test,and the alcohol precipitation process of the extract was optimized by single factor experiment and Box-Behnken design-response surface method.Results The opti-mal extraction process of the extract optimized by orthogonal test is as follows:10 times the amount of water(initial soaking for 0.5 h),boiling and extracting for 3 times,each time for 1.5 hrs.The optimal alcohol precipitation process of the extract optimized by single factor and response surface method is as follows:concentrating the medicinal solution to a relative density of 1.15(80℃),adjusting the endpoint alcohol concentration of the medicinal solution to 65%with 95%ethanol,and standing the alcohol for 48 hrs.Conclu-sion L_(9)(3^(4))orthogonal test combined with Box-Behnken design-response surface method used to optimize the extraction and alcohol precipitation process of hospital preparation Sanwei Banlan’gen granules can achieve the expected effect and provide reference for ac-tual expanded production.

关 键 词：三味板蓝根颗粒 L_(9)(3^(4))正交试验 单因素实验 Box-Behnken设计-响应面法 提取工艺 醇沉工艺 

分 类 号：R283.6[医药卫生—中药学]