Mosquito densovirus signifcantly reduces the vector susceptibility to dengue virus serotype 2 in Aedes albopictus mosquitoes(Diptera:Culicidae)  

作　　者：Ling Kong Jie Xiao Lu Yang Yuan Sui Duoquan Wang Shaoqiang Chen Peiwen Liu Xiao-Guang Chen Jinbao Gu 

机构地区：[1]Guangdong Provincial Key Laboratory of Tropical Disease Research,Depart-ment of Pathogen Biology,School of Public Health,Southern Medical Uni-versity,Guangzhou 510515,Guangdong,China [2]Brown School,Washington University,St.Louis,MO 63130,USA [3]National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention,Shanghai 200025,China [4]Shenzhen Aiming Pest Control Operation Service Company Limited,Shenz-hen,Guangdong,China

出　　处：《Infectious Diseases of Poverty》2023年第3期91-92,共2页贫困所致传染病（英文）

摘　　要：Background Dengue virus(DENV)is a major public health threat,with Aedes albopictus being the confrmed vector responsible for dengue epidemics in Guangzhou,China.Mosquito densoviruses(MDVs)are pathogenic mosquitospecifc viruses,and a novel MDV was previously isolated from Ae.albopictus in Guangzhou.This study aims to determine the prevalence of MDVs in wild Ae.albopictus populations and investigate their potential interactions with DENV and impact on vector susceptibility for DENV.Methods The prevalence of MDV in wild mosquitoes in China was investigated using open access sequencing data and PCR detection in Ae.albopictus in Guangzhou.The viral infection rate and titers in MDV-persistent C6/36 cells were evaluated at 12,24,48,72,96,and 120 h post infection(hpi)by indirect immunofuorescence assay(IFA)and real time quantitative PCR(RT-qPCR).The midgut infection rate(MIR),dissemination rate(DR),and salivary gland infection rate(SGIR)in various tissues of MDV-infected mosquitoes were detected and quantifed at 0,5,10,and 15 days post infection(dpi)by RT-PCR and RT-qPCR.The chi-square test evaluated dengue virus serotype 2(DENV-2)and Aedes aegypti densovirus(AaeDV)infection rates and related indices in mosquitoes,while Tukey’s LSD and t-tests compared viral titers in C6/36 cells and tissues over time.Results The results revealed a relatively wide distribution of MDVs in Aedes,Culex,and Anopheles mosquitoes in China and an over 68%positive rate.In vitro,signifcant reductions in DENV-2 titers in supernatant at 120 hpi,and an appar‑ent decrease in DENV-2-positive cells at 96 and 120 hpi were observed.In vivo,DENV-2 in the ovaries and salivary glands was frst detected at 10 dpi in both monoinfected and superinfected Ae.albopictus females,while MDV super‑infection with DENV-2 suppressed the salivary gland infection rate at 15 dpi.DENV-2 titer in the ovary and salivary glands of Ae.albopictus was reduced in superinfected mosquitoes at 15 dpi.Conclusions MDVs is widespread in natural mosquito populations,and replication

关 键 词：DENV-2 Mosquito densovirus SUPERINFECTION Aedes albopictus Vector susceptibility 

分 类 号：R384.1[医药卫生—医学寄生虫学]