大豆根瘤内生细菌的分离鉴定及其对大豆植株的促生效应  

Isolation and identification of endophytic bacteria from soybean nodule and their effects on soybean plant

作　　者：孙秀娟 徐伟慧[1] 王志刚[1] SUN Xiujuan;XU Weihui;WANG Zhigang(College of Life Science and Agriculture and Forestry,Qiqihar University,Qiqihar 161006,Heilongjiang,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006

出　　处：《浙江农业学报》2023年第7期1532-1541,共10页Acta Agriculturae Zhejiangensis

基　　金：黑龙江省重点研发计划项目(GA21B007,GZ20210067)。

摘　　要：为探究大豆根瘤内生细菌对大豆的促生效应,以大豆根瘤为实验材料,分离筛选根瘤内生细菌,测定其分泌植物激素能力及其对大豆株高、茎粗、干重、鲜重和根系生长方面的促生能力。结果表明:共筛选出17株根瘤内生细菌,经生理生化和16S rDNA鉴定,有10株为芽孢杆菌属(Bacillus),3株为巨大普里斯特氏菌属(Priestia),其余4株分别为肠杆菌属(Enterobacter)、克雷伯氏菌属(Klebsiella)、不动杆菌属(Acinetobacter)和农杆菌属(Agrobacterium)。通过溶血试验和16S rDNA鉴定筛选出10株促生能力强的菌株,其中,Klebsiella S8的吲哚乙酸(IAA)分泌量最高,为147.55 mg·L^(-1);赤霉素(GAs)分泌量最高的为Agrobacterium S10,分泌量达39.34 mg·L^(-1);Bacillus S3的铁载体活性最高,为70.57%;10株菌株处理的大豆株高、根长、干鲜重和茎粗均显著高于对照。综上所述,筛选的大豆根瘤内生细菌,对大豆具有较强的促进作用。To study the growth-promoting effect of endophytic bacteria in soybean root nodules on soybean growth,soybean root nodules were used as experimental materials to isolate and screen nodule endophytic bacteria,and their ability to secrete plant hormones and their promotion effect on soybean plant height,stem diameter,dry weight,fresh weight,and root growth were measured.The results showed that a total of 17 endophytic bacteria were screened out from root nodules.After physiological and biochemical analysis and 16S rDNA identification,10 strains belonged to Bacillus spp.,3 strains belonged to Priestia spp,and the remaining 4 strains belonged to Enterobacteriaceae,Klebsiella,Acinetobacter,and Agrobacterium.Through hemolysis test and 16S rDNA identification,10 strains with strong growth-promoting ability were selected.Among them,the IAA secretion of Klebsiella S8 was highest at 147.55 mg·L^(-1);the gibberellins of Agrobacterium S10 had the highest secretion at 39.34 mg·L^(-1);the siderophores activity of Bacillus S3 was the highest at 70.57%.The plant height,root length,dry fresh weight and stem diameter of soybean treated by these 10 strains were significantly higher than those of the control.In conclusion,the endophytic bacteria screened in this experiment had strong growth-promoting effect on soybean growth.

关 键 词：根瘤 内生细菌 大豆 溶血试验 促生能力 

分 类 号：S144.3[农业科学—肥料学] S565.1[农业科学—农业基础科学]