甜瓜SWEET基因家族的全基因组鉴定及生物信息学分析  

Genome-wide identification and bioinformatics analysis of SWEET gene family in melon

作　　者：寿伟松[1] 何艳军 沈佳[1] 许昕阳 SHOU Weisong;HE Yanjun;SHEN Jia;XU Xinyang(Institute of Vegetables,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江省农业科学院蔬菜研究所,浙江杭州310021

出　　处：《浙江农业学报》2023年第7期1591-1603,共13页Acta Agriculturae Zhejiangensis

基　　金：浙江省第三次全国农作物种质资源普查与收集行动(111821301354052030);浙江省农业(蔬菜新品种选育)新品种选育重大科技专项(2021C02065-3-2);国家自然科学基金(31872130)。

摘　　要：糖外排转运子(sugars will eventually be exported transporter,SWEET)是一类新型的糖转运蛋白,其在植物生长发育、植物与病原体互作以及植物胁迫耐受性方面都起着重要作用。基于甜瓜中还未有该家族基因的相关报道,本研究开展CmSWEET的全基因组鉴定和生物信息学分析。通过同源性搜索,总共鉴定到17个甜瓜CmSWEET基因。这些基因分布于甜瓜8条染色体上,具有典型的MtN3/saliva结构域。系统发育树将拟南芥、黄瓜和甜瓜的SWEET蛋白分为4个进化分支,其中亚族Ⅲ是甜瓜和黄瓜独有的亚族。基因启动子区域的顺式作用元件显示,CmSWEET含有许多与植物激素和应激相关的调节元件,暗示了CmSWEET在参与调节甜瓜生长发育以及环境适应性方面有重要作用。基因表达量分析表明,大部分CmSWEET基因在花(雌花和雄花)和根中高表达,部分CmSWEET基因在果实发育时期表达量较高。此外,RNA-Seq结果暗示,CmSWEET基因可能参与对尖孢镰刀菌和白粉病菌侵染的抗性响应。本研究为今后探究CmSWEET蛋白在甜瓜生长发育过程以及响应生物和非生物胁迫中的分子功能奠定了坚实的基础。Sugars will eventually be exported transporter(SWEET)is a novel family of sugar transporters that are involved in plant growth and development,plant-pathogen interaction,and stress tolerance.Due to the lack of reports of SWEET family genes in melon,this study carried out the genome-wide identification and bioinformatics analysis of CmSWEET.A total of 17 melon CmSWEET genes were identified by homology search.These genes that distributed on 8 chromosomes all have a typical MtN3/saliva domain.Phylogenetic analysis classified the SWEET proteins of Arabidopsis,cucumber and melon into four evolutionary clades,of which subfamilyⅢis unique to melon and cucumber.The cis-acting elements in the gene promoter region showed that CmSWEET contains many regulatory elements related to plant hormones and stress,suggesting that CmSWEET plays an important role in regulating growth and environmental adaptation in melon.Tissue-specific analysis showed that most of the CmSWEET genes were highly expressed in female and male flowers and roots,and some CmSWEET genes were highly expressed during fruit development.Furthermore,RNA-Seq results suggested that CmSWEET genes might be involved in resistance to Fusarium oxysporum and powdery mildew infection.This study lays a solid foundation for future research into their molecular function of CmSWEET in melon developmental processes and responses to biotic and abiotic stresses.

关 键 词：甜瓜 糖外排转运子 全基因组鉴定 进化分析 表达分析 

分 类 号：S652[农业科学—果树学]