萝卜春化响应相关基因鉴定及表达模式分析  

Genome-wide identification and expression analysis of key genes response to vernalization in radish ( Raphanus sativus )

作　　者：陈国户[1] 李广 温宏伟 尹倩 吴思文 王英 刘雪晴 赵龙龙 KHAN Afrasyab 桂尚枝 唐小燕 汪承刚[1,2] CHEN Guohu;LI Guang;WEN Hongwei;YIN Qian;WU Siwen;WANG Ying;LIU Xueqing;ZHAO Longlong;KHAN Afrasyab;GUI Shangzhi;TANG Xiaoyan;WANG Chenggang(Anhui Provincial Engineering Laboratory of Horticultural Crop Breeding,School of Horticulture,Anhui Agricultural University,Hefei 230036,China;Wanjiang Vegetable Industrial Technology Institute of Anhui Province,Ma anshan 238253,Anhui,China)

机构地区：[1]安徽省园艺作物育种工程实验室,安徽农业大学园艺学院,安徽合肥230036 [2]安徽省皖江蔬菜产业技术研究院,安徽马鞍山238253

出　　处：《浙江农业学报》2023年第7期1626-1637,共12页Acta Agriculturae Zhejiangensis

基　　金：安徽省教育厅科学研究项目(YJS20210248);国家自然科学基金(31801853);安徽省高校协同创新项目(GXXT-2021-054)。

摘　　要：抽薹开花是十字花科蔬菜最重要的性状之一。秋冬季节低温环境,使萝卜(Raphanus sativus L.)过早完成春化,导致未熟抽薹,严重影响产量与品质。本研究采用多重序列比对及系统进化树构建等生物信息学技术,鉴定出萝卜抽薹开花相关基因272个。对萝卜NHJS1、NHJS2和2#材料不同春化阶段的转录组数据进行重分析,筛选出春化相关基因;再结合META分析技术,共确定萝卜春化响应关键基因25个。利用蛋白质-蛋白质互作网络(PPI)分析了春化响应关键基因之间潜在的互作关系。对富集在春化、冷响应、赤霉素生物合成与信号转导途径上的春化响应关键基因进行了实时荧光定量PCR(qRT-PCR)分析,结果显示,RsGA20ox 1、RsAGL 18、RsFLC 2等基因在萝卜春化响应中发挥着重要作用。研究结果为深入解析萝卜春化分子机制奠定了基础。Bolting and flowering is an important character of Cruciferous crops.The vernalization would be completed in radish(Raphanus sativus L.)by low temperature environment in autumn and winter,which will be resulting in premature bolting.In this study,272 flowering related genes were identified by multiple sequence alignment and evolutionary tree analyses.The RNA-Seq sequencing data of radish varieties NHJS1,NHJS2 and 2#were analyzed,and 25 key vernalization responded genes were identified combined with the META analysis.The potential links between these key genes were estimated by the protein-protein interaction(PPI)network analysis.Furthermore,the key genes enriched in vernalization,cold responses,gibberellin biosynthesis and signal transduction pathway were verified by qRT-PCR,suggesting that RsGA20ox1,RsAGL18,RsFLC2 and other genes play important roles in vernalization.These results will lay a foundation for further understanding the molecular mechanism vernalization in radish.

关 键 词：萝卜 抽薹开花 春化响应 比较转录组 

分 类 号：S632.1[农业科学—蔬菜学]