针刺对穴“迎香-合谷”对变应性鼻炎大鼠Th1、Th2细胞因子及转录因子T-bet/GATA-3的影响  

Effect of acupuncture on acupoint"Yingxiang-Hegu"on Th1,Th2 cytokines and T-bet/GATA-3 of allergic rhinitis rats

作　　者：胡情 苏嘉琪 楼金成 苗镡允 尹浩 吉美奇 翟春涛 郝重耀 吕玉娥 HU Qing;SU Jia-qi;LOU Jin-cheng;MIAO Xin-yun;YIN Hao;JI Mei-qi;ZHAI Chun-tao;HAO Zhong-yao;LV Yu-e(Shanxi University of traditional Chinese Medicine,Jinzhong 030600,China;Shanxi Acupuncture Hospital,Taiyuan 030006,China)

机构地区：[1]山西中医药大学,山西晋中030600 [2]山西省针灸医院,山西太原030006

出　　处：《海南医学院学报》2023年第15期1135-1143,共9页Journal of Hainan Medical University

基　　金：吕景山针药结合科技创新团队(2019TD⁃003);吕景山针灸与生物电子医学创新团队(2022TD⁃1006)。

摘　　要：目的:探讨“迎香-合谷”对穴对过敏性鼻炎大鼠Th1、Th2相关细胞因子及转录因子T-bet、GATA-3的影响。方法:大鼠随机分成空白组、模型组、对穴组3组。建立卵清蛋白(OVA)AR大鼠模型,观察大鼠一般情况并进行行为学评分,造模成功后第2天开始对对穴组行针刺干预,1次/d,20 min/次,共10 d。干预结束后观察大鼠一般行为、行为学评分及鼻黏膜组织形态学改变,鼻腔灌洗液涂片染色后镜检计数嗜酸性粒细胞(EOS),ELISA检测大鼠血清总IgE、IFN-γ、IL-12、IL-4、IL-5含量,Western blot法和免疫组化法(IHC)检测大鼠鼻黏膜组织特异性转录因子T-bet、GATA-3蛋白水平和阳性蛋白的表达情况。结果:造模后,除空白组外,模型组、对穴组大鼠行为学观察评分均大于5分,提示造模成功。针刺对穴“迎香-合谷”干预后,对穴组、西药组大鼠行为学评分较模型组明显下降(P<0.05)。镜检示模型组鼻黏膜结构明显破坏,纤毛排列不连续,参差不齐,局部充血肿胀,上皮细胞大量脱落坏死,杯状细胞增生,有明显炎症细胞浸润。对穴组鼻黏膜病理程度较模型组明显减轻。与模型组比较,经对穴针刺干预后大鼠血清中的IFN-γ、IL-2、IL-12明显升高(P<0.05),而IgE、IL-4、IL-5、IL-6明显降低(P<0.05),鼻黏膜GATA-3蛋白及阳性表达明显降低,T-bet明显升高(P<0.05)。结论:针刺对穴“迎香-合谷”能有效改善AR大鼠鼻部敏感症状、达到控制鼻部炎症的目的,其机制可能是针刺对穴干预后上调了T-bet的表达,降低GATA-3水平,并促进Th1型细胞因子的生成,抑制Th2细胞的合成与分泌,从而使Th1、Th2恢复免疫平衡状态。objective:To explore the effect of"Yingxiang-Hegu"on Th1,Th2-related cytokines and transcription factors T-bet and GATA-3 in rats with allergic rhinitis.Methods:Rats were randomly divided into three groups:blank group,model group and acupoint group.The rat model of ovalbumin(OVA)AR was established,and the general condition of the rats was ob-served and scored.Acupuncture intervention was performed on the acupoint group on the second day after successful modeling,once per day for 20 minutes for 10 days.After intervention,the general behavior,behavioral score and histomorphological changes of nasal mucosa were observed.Eosinophils(EOS)were counted under microscope after nasal lavage smear staining,and the con-tents of total IgE,IFN-γ,IL-12,IL-4 and IL-5 in serum were detected by ELISA.Westernblot and IHC were used to detect the protein level and positive protein expression of specific transcription factors T-bet and GATA-3 in rat nasal mucosa.Results:Af-ter the establishment of the model,except for the blank group,the behavioral observation scores of rats in the model group and acupoint group were more than 5 points,indicating that the model was successful.After acupuncture intervention on acupoint"Yingxiang-Hegu",the behavioral score of rats in the acupoint group and western medicine group was significantly lower than that in the model group(P<0.05).Microscopic examination showed that the structure of nasal mucosa in the model group was obvi-ously damaged,cilia were arranged discontinuously,uneven,local congestion and swelling,a large number of epithelial cells exfo-liated and necrotic,goblet cell proliferation,obvious inflammatory cell infiltration.The pathological degree of nasal mucosa in the pair point group was significantly less than that in the model group.Compared with the model group,the levels of IFN-γ,IL-2 and IL-12 in serum were significantly increased,while IgE,IL-4,IL-5 and IL-6 were significantly decreased,GATA-3 protein and positive expression in nasal mucosa were significantly decreased and T-be

关 键 词：变应性鼻炎 对穴 “迎香”穴 “合谷”穴 辅助性T细胞 细胞因子 GATA结合蛋白3 T盒子转录因子 

分 类 号：R245[医药卫生—针灸推拿学]