草莓茎尖组织培养育苗技术的培养基优化  

Optimization for Tissue Culture Cultivated Seedling Technology of Strawberry Stem Tip

作　　者：王禹[1] 牟蕴慧[1] 侯睿宁 刘万达[1] 谭巍[1] 于飞[1] WANG Yu;MU Yunhui;HOU Ruining;LIU Wanda;TAN Wei;YU Fei(Horticultural Branch,Heilongjiang Academy of Agricultural Sciences,Harbin 150069,China)

机构地区：[1]黑龙江省农业科学院园艺分院,黑龙江哈尔滨150069

出　　处：《黑龙江农业科学》2023年第8期72-77,共6页Heilongjiang Agricultural Sciences

基　　金：黑龙江省农业科学院院级科研项目(2020YYYF044)。

摘　　要：草莓茎尖组织培养育苗技术通常采用诱导分化、增殖、生根3种培养基进行培养,工序较为繁琐,为探索出一种既可用于草莓茎尖诱导分化,也可用于增殖、生根培养步骤的通用培养基,进一步简化操作工序,以草莓“红颜”0.3 mm的茎尖为试材,生长激素采用6-BA、TDZ、NAA、IAA、IBA并分别设置5个不同浓度,应用L_(25)(5^(6))的正交试验,筛选出可用于茎尖诱导分化、增殖、生根培养的通用培养基为MS+6-BA 0.3 mg·L^(-1)+TDZ 0.7 mg·L^(-1)+NAA 0.1 mg·L^(-1)+IAA 0.1 mg·L^(-1)+IBA 0.3 mg·L^(-1),诱导分化率为67.2%,增殖系数为8.5,生根率为95.1%。Strawberry stem tip tissue culture and seedling cultivation techniques usually use three types of culture media of induction differentiation,proliferation,and rooting,and the process is relatively complicated.In order to explore a universal culture medium that can be used for both strawberry stem tip induction differentiation,proliferation,and rooting cultivation steps,and further simplify the operation process,the 0.3 mm stem tips of strawberry'Red Face'were used as test material,and growt h hormones were used with 6-BA,TDZ,NAA,IAA and IBA at five different concentrations,and the orthogonal experiment of L_(25)(5^(6))was applied.The general culture medium for inducing differentiation,proliferation,and rooting culture of stem tips was selected as MS+6-BA 0.3 mg·L^(-1)+TDZ 0.7 mg·L^(-1)+NAA 0.1 mg·L^(-1)+IAA 0.1 mg·L^(-1)+IBA 0.3 mg·L^(-1).The induced differentiation rate was 67.2%,the value added coefficient was 8.5,and the rooting rate was 95.1%.

关 键 词：组织培养 育苗 正交试验 培养基优化 

分 类 号：S668.4[农业科学—果树学]