Decellularized extracellular matrix particle-based biomaterials for cartilage repair applications  

作　　者：Peng Guo Nan Jiang Carina Mini Gregor Miklosic Songsong Zhu Andrea J.Vernengo Matteo D’Este Sibylle Grad Mauro Alini Zhen Li 

机构地区：[1]AO Research Institute Davos,Davos 7270,Switzerland [2]Department of Spine Surgery,The Sixth Affiliated Hospital,Sun Yat-sen University,Guangzhou 510655,China [3]State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Disease and West China Hospital of Stomatology,Sichuan University,Chengdu 610041,China [4]Institute for Biomechanics,ETH Zürich,Zürich 8093,Switzerland

出　　处：《Journal of Materials Science & Technology》2023年第29期194-203,共10页材料科学技术（英文版）

基　　金：AO Foundation and AOSpine Inter-national.Peng Guo and Nan Jiang were funded by Sino Swiss Sci-ence and Technology Cooperation Program(Nos.EG-CN_01-032019 and EG-CN_04-042018);China Scholarship Council.MD and GM gratefully acknowledge funding from the Swiss National Sci-ence Foundation(SNSF,No.310030E_189310).

摘　　要：Cartilage Decellularized ExtraCellular Matrix(dECM)materials have shown promising cartilage regenera-tion capacity due to their chondrogenic bioactivity.However,the limited retention of ECM components and the reduced integrity of functional ECM molecules during traditional decellularization processes im-pair the biomimicry of these materials.The current study aims to fabricate biomimetic materials con-taining decellularized cartilage particles that have an intact molecular structure and native composition as biomaterial inks and hydrogels for cartilage repair.For this,we established a novel two-fraction de-cellularization strategy for the preparation of reconstituted dECM(rdECM)particles by mixing the two-fraction components,as well as a one-fraction decellularization strategy for the preparation of biomimetic dECM(bdECM)particles.Hyaluronic acid-tyramine(THA)hydrogels containing rdECM or bdECM particles were produced and characterized via rheological test,swelling and stability evaluation,and compression test.The results showed that our novel decellularization strategies preserved intact proteoglycans and collagen at a higher retention rate with adequate DNA removal compared to traditional methods of de-cellularization.The addition of rdECM or bdECM particles significantly increased the shear moduli of the THA bioinks while preserving their shear-thinning properties.bdECM particle-embedded THA hydrogels also achieved long-term stability with a swelling ratio of 70%and high retention of glycosaminoglycans and collagen after long-term incubation,while rdECM particle-embedded THA hydrogels showed unsat-isfactory stability as self-standing biomaterials.Compared to pure THA hydrogels,the addition of bdECM particles significantly enhanced the compression moduli.In summary,our decellularization methods are successful in the retention of functional and intact cartilage components with high yield.Both rdECM and bdECM particles can be supplemented in THA bioinks for biomimetic cartilage 3D printing.Hydro-gels with cartila

关 键 词：Cartilage tissue engineering Decellularized extracellular matrix Proteoglycan integrity Biomimetic hydrogels Biomaterial Bioink 

分 类 号：R318.08[医药卫生—生物医学工程]