藏猪和大约克猪FABP3基因多态性及差异表达分析  

Polymorphisms and Expressions of FABP3 in Tibetan and Yorkshire Pigs

作　　者：杨绍康 薄素雪 韩雨晴 叶幼荣 商鹏 强巴央宗 YANG Shaokang;BO Suxue;HAN Yuqing;YE Yourong;SHANG Peng;CHAMBA Yangzom(College of Animal Science,Tibet Agriculture and Animal Husbandry University,Linzhi,Tibet 860000,China;Provincial and Ministerial Co-founded Collaborative Innovation Center for R&D on Tibet Characteristic Agricultural and Animal Husbandry Resources,Linzhi,Tibet 860000,China)

机构地区：[1]西藏农牧学院动物科学学院,西藏林芝860000 [2]西藏特色农牧资源研发省部共建协同创新中心,西藏林芝860000

出　　处：《福建农业学报》2023年第6期639-645,共7页Fujian Journal of Agricultural Sciences

基　　金：西藏自治区重大科技专项(XZ202101ZD0005N);国家自然科学基金地区科学基金项目(32160773);中国农业大学基本科研业务费专项(2022TC002)。

摘　　要：【目的】脂肪酸结合蛋白3(FABP3)参与长链脂肪酸的摄取及利用,在脂肪沉积中发挥重要作用。探究FABP3基因在藏猪和大约克猪间的基因多态性和表达差异可为藏猪品质改良提供分子机制参考。【方法】选取180日龄藏猪和大约克猪为研究对象,对两猪种FABP3基因5'侧翼区和CDS区进行单核苷酸多态性(SNPs)筛选,使用实时荧光定量检测FABP3基因在肝脏、背最长肌和背脂3个组织中的表达量。【结果】在FABP3基因5'侧翼区筛选到T-114C和C-635A等2个SNPs位点,且SNPs位点基因型频率在藏猪与大约克猪群体间均呈极显著差异(P<0.01);经转录因子预测发现这2个SNPs位点与前脂肪细胞的更新、分化以及脂肪沉积相关,推测这两个多态性位点是参与调控FABP3基因表达的重要功能位点。FABP3基因在藏猪肝脏、背最长肌中的表达量极显著高于大约克猪(P<0.01),在背脂中的表达量显著高于大约克猪(P<0.05)。【结论】推测FABP3基因可能为调控藏猪脂肪代谢的重要候选基因,在藏猪脂肪沉积中呈正向调控作用。【Objective】Fatty acid binding protein 3(FABP3)is involved in the uptake and utilization of long-chain fatty acids and plays an important role in fat deposition.To investigate the gene polymorphism and expression differences of FABP3 gene between Tibetan and York pigs could help improve the quality of Tibetan pigs for the genetic level.【Methods】In this study,single nucleotide polymorphisms(SNPs)in the 5'flanking region and CDS region of FABP3 in randomly selected 180-d-old Tibetan and Yorkshire pigs were tested.Expressions of FABP3 in the liver,longest dorsal muscle,and dorsal fat were detected using real-time fluorescence.【Results】Two SNPs,T-114C and C-635A,were found in FABP3 with significantly differentiated genotype frequencies between the two species of pigs(P<0.01).Upon transcription factor prediction,these 2 SNPs loci were found to be associated with preadipocyte renewal,differentiation,and fat deposition,and it was hypothesized that they were important functional loci involved in the regulation of FABP3 gene expression.The expressions of FABP3 in the liver and longest dorsal muscle of Tibetan pigs were extremely significantly higher than those of Yorkshire pigs(P<0.01),and that in the dorsal fat significantly higher than that of Yorkshire pigs(P<0.05).【Conclusion】FABP3 might be closely related to the regulation of fat metabolism and deposition of Tibetan pigs which differs from Yorkshire variety.

关 键 词：藏猪 FABP3基因 脂肪沉积 多态性位点 

分 类 号：S813.1[农业科学—畜牧学]