贝莱斯芽孢杆菌的鉴定和抑菌作用研究  

Identification and Antimicrobial Test of Bacillus Velezensis

作　　者：周淑贞 辜质纯 张瑞雪 廖洁丹 谢志恒 张悻 顾万军 黄良宗 ZHOU Shuzhen;GU Zhichun;ZHANG Ruixue;LIAO Jiedan;XIE Zhiheng;ZHANG Xing;GU Wanjun;HUANG Liangzong(Eastern Along Pharmaceutical Co.,Ltd.,Nanhai,Foshan Guangdong 528234;Foshan Nanhai District Weide Bio-Technology Co.,Ltd,Guangdong 528234;Foshan University School of Life Science and Engineering,Guangdong 528231)

机构地区：[1]佛山市南海东方澳龙制药有限公司,广东佛山528234 [2]佛山市南海区维德生物技术有限公司,广东佛山528234 [3]佛山科学技术学院生命科学与工程学院,广东佛山528231

出　　处：《广东畜牧兽医科技》2023年第4期30-34,41,共6页Guangdong Journal of Animal and Veterinary Science

基　　金：广东省现代草牧业(羊)产业技术体系项目(2019KJ127)。

摘　　要：该研究对6株贝莱斯芽孢杆菌分离株进行测序鉴定、药敏试验与抑菌试验。结果表明:所分离的6株菌16SrDNA序列与贝莱斯芽孢杆菌处于同一遗传进化树分支,对选定的7种抗菌药物均敏感。应用指示菌进行抑菌试验,6株菌及其代谢产物对金黄色葡萄球菌和大肠杆菌均有抑菌作用,抑菌作用从强到弱依次是:贝莱斯芽孢杆菌菌液、上清液和菌液滤液。In this study,sequencing identification,drug sensitivity testing,and antibacterial assays were conducted on six Bacillus velezensis strains isolated from diverse sources.The results indicated that the 16S rDNA sequences of these six isolates were positioned within the same genetic evolutionary branch of Bacillus velezensis,and sensitivity to seven selected antimicrobial drugs was exhibited.Bacteriostatic effects against both Staphylococcus aureus and Escherichia coli were demonstrated for the six strains and their metabolites.The bacteriostatic intensity of the samples followed the order of Bacillus velezensis liquid,supernatant,and filtrate.

关 键 词：贝莱斯芽孢杆菌 鉴定 抑菌试验 

分 类 号：S815.1[农业科学—畜牧学]