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作 者:朱键[1] 陈天天 刘智勇[1] 郑常龙[1] Zhu Jian;Chen Tiantian;Liu Zhiyong;Zheng Changlong(Department of Emergency,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China)
机构地区:[1]中山大学附属第三医院急诊科,广州510630 [2]中山大学附属第三医院心胸外科,广州510630
出 处:《新医学》2023年第8期554-559,共6页Journal of New Medicine
基 金:国家自然科学基金(81500287)。
摘 要:目的研究过表达微RNA-124(miR-124)对肺动脉高压大鼠肺血管重构的影响及初步探索其可能机制。方法使用野百合碱(MCT)经腹腔注射建立大鼠肺动脉高压模型,气管内滴注腺相关病毒(AAV)介导的miR-124进行干预,记录大鼠心脏右室收缩压(RVSP)、平均动脉压和心率,实时荧光定量PCR法检测大鼠肺组织中miR-124的表达,van Gieson染色观察肺内小动脉的重构程度并计算血管外径在25~50μm以及51~100μm的肺小动脉的中膜/外径比值,蛋白免疫印迹法检测肺组织中IL-6与p-STAT3的蛋白水平。结果miR-124在肺动脉高压大鼠肺组织中呈低表达;过表达miR-124可导致肺动脉高压大鼠RVSP下降,肺内小动脉中膜/外径明显降低,肺组织中IL-6和p-STAT3的表达下调。结论AAV介导的miR-124过表达可明显减轻肺动脉高压的大鼠的RVSP以及肺血管重构,其机制可能与下调IL-6和p-STAT3的表达有关。Objective To evaluate the effect of overexpression of microRNA-124(miR-124)on pulmonary vascular remodeling,and to unravel the potential mechanism in rats with pulmonary arterial hypertension(PAH).Methods In the monocrotaline(MCT)-induced PAH rat models,intratracheal infusion of miR-124 carried by adeno-associated virus(AAV)was performed.The right ventricular systolic blood pressure(RVSP),mean systolic arterial pressure and heart rate were recorded.The expression level of miR-124 in lung tissues of rats was detected by real-time quantitative PCR.The histological morphology of pulmonary arterioles was observed by van Gieson staining.The ratios of medium to outside diameter of pulmonary arterioles with vessel diameters in 25-50μm and 51-100μm were calculated.The expression levels of IL-6 and p-STAT3 protein in lung tissues were detected by Western blot.Results The expression level of miR-124 was down-regulated in pulmonary tissues of rats with PAH.RVSP was significantly decreased,the ratios of medium to outside diameter of pulmonary arterioles were significantly attenuated,and the expression levels of IL-6 and p-STAT3 proteins were significantly down-regulated by overexpression of miR-124.Conclusion AAVmediated overexpression of miR-124 can significantly reduce RVSP and pulmonary vascular remodeling in rats with PAH,probably by down-regulating the expression levels of IL-6 and p-STAT3.
分 类 号:R544.1[医药卫生—心血管疾病]
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