miR515、ITLN2蛋白在子宫内膜异位症患者子宫内膜组织中的表达及意义分析  

Expression and significance of miR515 and ITLN2 protein in endometrial tissues of patients with endometriosis

作　　者：刘庄[1] 孙颖 王钦[1] 张琳 李泽武 LIU Zhuang;SUN Ying;WANG Qin(Department of Reproductive Medicine,Affiliated Hospital of Jining Medical College,Jining Shandong 272029,China;Department of Anesthesiology,Affiliated Hospital of Jining Medical College,Jining Shandong 272029,China)

机构地区：[1]济宁医学院附属医院生殖医学科,山东济宁272029 [2]济宁医学院附属医院麻醉科,山东济宁272029

出　　处：《临床和实验医学杂志》2023年第14期1522-1526,共5页Journal of Clinical and Experimental Medicine

基　　金：国家自然科学基金青年基金(编号:81701526);济宁市科技局重点研发项目(编号:2022YXNS086)。

摘　　要：目的 探讨子宫内膜异位症(EMT)患者子宫内膜组织中微小RNA-515(miR-515)、内凝集蛋白2(ITLN2)的表达及其意义。方法 回顾性选取2018年8月至2022年6月济宁医学院附属医院生殖医学科收集的80例EMT异位内膜(异位组)、80例子宫内膜腺肌症在位内膜(在位组)、80例正常子宫内膜(对照组)。采用苏木精-伊红染色对比3组的子宫内膜病理学差异,采用RT-PCR技术检测3组病理组织中的miR-515表达,采用蛋白质印迹法检测3组标本中的ITLN2蛋白表达情况。并对异位组患者按照疾病类型(卵巢型56例,其他类型24例)、美国生育协会修正EMT分期(r-AFSⅠ+Ⅱ患者30例,Ⅲ+Ⅳ患者50例)进行分组,对比异位组的miR-515、ITLN2表达差异。结果 异位组和在位组内膜组织失去正常形态结构,细胞核丰富。异位组的子宫内膜组织中miR-515相对表达强度为0.531±0.112,显著高于在位组(0.209±0.084)和对照组(0.096±0.032),且在位组的miR-515相对表达强度显著高于对照组,差异均有统计学意义(P<0.05)。异位组的子宫内膜组织中ITLN2蛋白表达强度为0.276±0.095,显著低于在位组(0.514±0.134)和对照组(0.692±0.162),且在位组的ITLN2蛋白表达强度显著低于对照组,差异均有统计学意义(P<0.05)。卵巢型EMT患者子宫内膜组织中的miR-515相对表达强度为0.603±0.104,显著高于其他类型(0.363±0.095),ITLN2蛋白表达强度为0.211±0.089,显著低于其他类型(0.428±0.091),差异均有统计学意义(P<0.05)。r-AFS分期Ⅲ+Ⅳ期的EMT患者子宫内膜组织中的miR-515相对表达强度为0.598±0.095,显著高于r-AFS分期Ⅰ+Ⅱ期(0.419±0.109),ITLN2蛋白表达强度为0.251±0.088,显著低于r-AFS分期Ⅰ+Ⅱ期(0.318±0.090),差异均有统计学意义(P<0.05)。结论 EMT患者子宫内膜组织中miR-515表达上调,ITLN2蛋白表达下调,其表达变化可能与疾病分型、r-AFS分期有关,有助于EMT患者的诊断和治疗策略的制定。Objective To investigate the expression and significance of microRNA-515(miR-515)and internal lectin 2(ITLN2)in endometrial tissues of patients with endometriosis(EMT).Methods Eighty cases of EMT ectopic endometrium(ectopic group),80 cases of eutopic endometrium of EMT adenomyosis(eutopic group),and 80 cases of normal endometrium(control group)collected by Department of Reproductive Medicine of Affiliated Hospital of Jining Medical University from August 2018 to June 2022 were retrospectively seleted.The pathological differences in the endometrium of the three groups were compared by hematoxylin and eosin staining.The expression of miR-515 in the three groups of pathological tissues was detected by RT-PCR technology,and the expression of ITLN2 protein in the three groups of specimens was detected by Western blotting.Patients in eutopic group were divided by disease type(56 patients with ovarian type and 24 patients with other types)and the American Fertility Society,EMT stage(r-AFS,30 patients inⅠ+Ⅱ,50 patients inⅢ+Ⅳ),the expression differences of miR-515 and ITLN2 in the ectopic group were compared.Results The endometrium tissue in the ectopic and eutopic groups loses its normal morphology and structure,with abundant nuclei.The relative expression intensity of miR-515 in endometrial tissues of ectopic group was 0.531±0.112,which was significantly higher than that of in-position group(0.209±0.084)and control group(0.096±0.032),and the relative expression intensity of miR-515 in in-position group was significantly higher than that of control group,the differences were statistically significant(P<0.05).The expression intensity of ITLN2 protein in endometrial tissues of ectopic group was 0.276±0.095,which was significantly lower than that of in-position group(0.514±0.134)and control group(0.692±0.162),and the expression intensity of ITLN2 protein in in-position group was significantly lower than that of control group,the differences were statistically significant(P<0.05).The relative expression intensi

关 键 词：子宫内膜异位症 微小RNA-515 内凝集蛋白2 疾病分型 r-AFS分期 

分 类 号：R711.71[医药卫生—妇产科学]