金钱草不同部位多元化学成分的分析与评价  

Analysis and Evaluation of Multiple Chemical Constituents in Different Parts of Lysimachiae Herba

作　　者：周永逸 薛佳 陈海杰 张月婵[2] 吴楠 袁嘉欢 石婧婧 邹立思[1] 刘训红[1,3] 杨薇 程建明 ZHOU Yong-yi;XUE Jia;CHEN Hai-jie;ZHANG Yue-chan;WU Nan;YUAN Jia-huan;SHI Jing-jing;ZOU Li-si;LIU Xun-hong;YANG Wei;CHENG Jian-ming(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Zhangjiagang Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine,Zhangjiagang 215600,China;Jiangsu Province Engineering Research Center of Classical Prescription,Nanjing 210023,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]南京中医药大学附属张家港医院,江苏张家港215600 [3]江苏省经典名方工程研究中心,江苏南京210023

出　　处：《分析测试学报》2023年第8期930-942,共13页Journal of Instrumental Analysis

基　　金：江苏高校品牌专业建设工程资助项目(PPZY2015A070);苏州市科技发展计划(SYSD2020196)。

摘　　要：基于超高效液相色谱-三重四极杆/线性离子阱质谱(UHPLC-Triple Quad-MS/MS)建立了同时测定金钱草中黄酮类、有机酸类、核苷酸类及氨基酸类等多元化学成分含量的方法,并结合多元统计分析对金钱草不同部位(茎、叶、花及果实)的化学成分进行分析与评价。采用Agilent Extend-C_(18)(2.1 mm×100 mm,1.8µm)色谱柱,以0.1%甲酸(A)和甲醇(B)作为流动相进行梯度洗脱,流速0.30 mL/min,多反应监测模式(MRM)检测。根据40种目标成分的含量,采用聚类分析(HCA)、主成分分析(PCA)、偏最小二乘判别分析(PLS-DA)、单因素方差分析(One-way ANOVA)和灰色关联度分析(GRA)对金钱草不同部位进行综合评价。结果显示,40种成分的色谱峰分离度良好,在相应的质量浓度范围内,标准曲线的相关系数(r^(2))均不低于0.9992,检出限(LOD)为0.21~19.76 ng/mL,定量下限(LOQ)为0.71~65.88 ng/mL,平均加标回收率为99.1%~102%,相对标准偏差(RSD)不大于2.9%。金钱草不同部位中40种化学成分含量的差异显著,其中花、叶的综合质量较好。该研究建立的评价体系准确、可靠,可用于金钱草药材内在质量的综合评价,为金钱草药材的合理采收和综合开发利用提供了科学依据。A method based on ultra high performance liquid chromatography-triple quadrupole lin-ear ion trap mass spectrometry(UHPLC-Triple Quad-MS/MS)was developed for the simultaneous determination of flavonoids,organic acids,nucleosides and amino acids in the stems,leaves,flow-ers and fruits of Lysimachiae Herba,and multivariate statistical analysis was used to compare the dif-ferent parts of Lysimachiae Herba.The preparation conditions were optimized by single factor test.The samples were separated on an Agilent Extend-C_(18)(2.1 mm×100 mm,1.8µm)analytical col-umn at 30℃by gradient elution,with 0.1%formic acid-methanol as mobile phase at a flow rate of 0.30 mL/min,and analyzed under multiple-reaction monitoring(MRM)mode.Based on the results of content,the different parts of Lysimachiae Herba were comprehensively evaluated by the combina-tion of hierarchical cluster analysis(HCA),principal component analysis(PCA),partial least squares discriminant analysis(PLS-DA),one-way analysis of variance(One-way ANOVA)and grey relation analysis(GRA).The optimal extraction conditions were using 80%methanol as the ex-traction solvent,with solid-liquid ratio of 1∶10,and ultrasonic extraction of 60 min at room tem-perature.The results showed that the peaks for 40 constituents were sucessfully separated,with their correlation coefficients(r^(2))not less than 0.9992.The limits of detection(LODs)and limits of quanti-tation(LOQs)were in the range of 0.21-19.76 ng/mL and 0.71-65.88 ng/mL,respectively.The average recoveries ranged from 99.1%to 102%,with RSDs not more than 2.9%.HCA and PCA could be used to discriminate samples of different parts,and gather stems,leaves,flowers,and fruits into one category,respectively,indicating that the same samples from different batches had stable quality.As could be seen from the heat map,there were fewer constituents with high content in the stem,and more constituents with high content in the leaves and flowers.Under the condition that VIP was greater than 1,PLS-DA screened a total of 13 important c

关 键 词：金钱草 不同部位 超高效液相色谱-三重四极杆/线性离子阱质谱(UHPLC-Triple Quad-MS/MS) 多元化学成分 多元统计分析 

分 类 号：O657.63[理学—分析化学] TQ460.72[理学—化学]