不同离心参数对小剂量全血白膜法采血袋制备富血小板血浆回收率的影响  

Effects of different centrifugation parameters on the recovery rate of platelet-rich plasma prepared by small-dose whole blood buffy coat method blood collection bag

作　　者：张彬[1] 陆华 宋娜丽 王世春 刘奇[1] 吴春曦 易中梅 ZHANG Bin;LU Hua;SONG Nali;WANG Shichun;LIU Qi;WU Chunxi;YI Zhongmei(Department of Blood Transfusion,The First Affiliated Hospital of Army Medical University/Chongqing Blood Bank of PLA,Chongqing 400038,China;Department of Blood Transfusion,The Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China)

机构地区：[1]陆军军医大学第一附属医院输血科/中国人民解放军重庆血站,重庆400038 [2]重庆医科大学附属第二医院输血科,400010

出　　处：《重庆医学》2023年第15期2301-2304,共4页Chongqing medicine

基　　金：中国人民解放军军队护理创新与培育专项(2021HL012)。

摘　　要：目的比较小剂量全血白膜法采血袋制备富血小板血浆(PRP)的回收率。方法相同来源的全血50、100 mL在不同离心参数下离心,手工分离白膜,比较白膜层血小板回收率;将白膜10、20 mL在不同离心参数下离心,分离PRP,比较PRP血小板回收率;对PRP进行血小板低渗休克反应(HSR),验证血小板功能。结果第1次离心,50、100 mL全血分别在470、940 g离心11 min白膜层血小板回收率即达到较高水平[(73.50±13.20)%、(71.30±11.12)%]。第2次离心,10、20 mL白膜分别在70、100 g离心13 min PRP血小板回收率达到最高水平[(95.07±4.90)%、(85.15±2.28)%]。10 mL白膜在70 g离心13 min、100 g离心9 min PRP HSR比率达到最高水平[(53.26±3.61)%、(55.50±3.94)%];20 mL白膜在70 g离心17 min、100 g离心13 min PRP HSR比率达到最高水平[(59.53±6.60)%、(59.39±4.10)%]。结论小剂量全血白膜法制备PRP可获得理想的回收率,是现有制备剂量的补充。Objective To compare the recovery rate of platelet-rich plasma(PRP)prepared using a small-dose whole blood buffy coat blood collection bag.Methods 50 mL and 100 mL of whole blood from the same source were centrifuged using different centrifugation parameters.The buffy coat was then manually separated,and the recovery rate of platelets in the buffy coat was compared.10 mL and 20 mL of buffy coat were centrifuged using different centrifugation parameters to separate PRP,and the recovery rate of PRP platelets was compared.Platelet hypotonic shock response(HSR)was performed on PRP to assess platelet function.Results In the first centrifugation,50 mL and 100 mL of whole blood were centrifuged at 470 g and 940 g for 11 minutes,respectively.The recovery rate of buffy coat platelets reached a high level,with values of(73.50±13.20)%and(71.30±11.12)%for the two samples.In the second centrifugation,10 mL and 20 mL of the buffy coat were centrifuged at 70 g and 100 g for 13 minutes,respectively.The recovery rate of PRP platelets reached its highest level at(95.07±4.90)%and(85.15±2.28)%.The ratio of PRP HSR reached its highest level[(53.26±3.61)%,(55.50±3.94)%]when 10 mL of buffy coat was centrifuged at 70 g for 13 minutes and 100 g for 9 minutes.When a 20 mL buffy coat was centrifuged at 70 g for 17 minutes and 100 g for 13 minutes,the ratio of PRP HSR reached its highest level[(59.53±6.60)%,(59.39±4.10)%].Conclusion The preparation of PRP by small-dose whole blood buffy coat method can achieve an optimal recovery rate,making it a valuable addition to the current preparation methods.

关 键 词：全血 制备 白膜 富血小板血浆 比率 

分 类 号：R457.1[医药卫生—治疗学]