Myelin histology:a key tool in nervous system research  

作　　者：Óscar Darío García-García Víctor Carriel Jesús Chato-Astrain 

机构地区：[1]Department of Histology,Tissue Engineering Group,University of Granada&Instituto de Investigación Biosanitaria ibs.GRANADA,Granada,Spain

出　　处：《Neural Regeneration Research》2024年第2期277-281,共5页中国神经再生研究（英文版）

基　　金：supported by the Spanish“Plan Nacional de Investigación Científica,Desarrollo e Innovación Tecnológica,Ministerio de Economía y Competitividad(Instituto de Salud CarlosⅢ)”,Grant FIS PI20-0318 co-financed by“Fondo Europeo de Desarrollo Regional ERDF-FEDER European Union”;Grant P18-RT-5059“Plan Andaluz de Investigación,Desarrollo e Innovación(PAIDI 2020),Consejería de Transformación Económica,Industria,Conocimiento y Universidades,Junta de Andalucía,Espana”(all to VC);Grant PPJIA202219“Ayudas del plan propio UGR 2022,Plan propio de investigación y transferencia,Universidad de Granada,Espana”(to JCA andóDGG)。

摘　　要：The myelin sheath is a lipoprotein-rich,multilayered structure capable of increasing conduction velocity in central and peripheral myelinated nerve fibers.Due to the complex structure and composition of myelin,various histological techniques have been developed over the centuries to evaluate myelin under normal,pathological or experimental conditions.Today,methods to assess myelin integrity or content are key tools in both clinical diagnosis and neuroscience research.In this review,we provide an updated summary of the composition and structure of the myelin sheath and discuss some histological procedures,from tissue fixation and processing techniques to the most used and practical myelin histological staining methods.Considering the lipoprotein nature of myelin,the main features and technical details of the different available methods that can be used to evaluate the lipid or protein components of myelin are described,as well as the precise ultrastructural techniques.

关 键 词：fluorescence microscopy HISTOLOGY light microscopy lipid histochemistry metallographic techniques myelin histochemistry myelin immunohistochemistry myelin structure&composition myelin ultrastructural evaluation tissue fixation&processing 

分 类 号：R338[医药卫生—人体生理学]