烟草NtCycB2基因通过茉莉酮酸途径调控腺毛发生  

Tobacco NtCycB2 regulates glandular trichomes generation via jasmonic acid pathway

作　　者：徐寒池 徐梦晓 滕环瑜 睢雅琳 张洪映[1] 崔红[1] XU Hanchi;XU Mengxiao;TENG Huanyu;SUI Yalin;ZHANG Hongying;CUI Hong(College of Tobacco Science,Henan Agricultural University)

机构地区：[1]河南农业大学烟草学院,河南郑州450002

出　　处：《中国烟草学报》2023年第4期96-104,共9页Acta Tabacaria Sinica

基　　金：中国烟草总公司科技重大专项项目“烤烟叶面化学成分的分子调控和定向改良”[110202101005(JY-05)];河南省自然科学基金项目“腺毛特异表达元件的鉴定、应用及其响应调控机制的研究”(222300420452)。

摘　　要：【目的】为了探究烟草NtCycB2调控腺毛发生的可能分子机制。【方法】采用酶联免疫吸附法(ELISA)对K326、NtCycB2过表达株系NtCycB2-M和NtCycB2敲除株系NtCycB2-OE叶片内源激素进行测定;通过实时定量PCR分析NtCycB2对茉莉酮酸(JA)合成和信号途径相关基因表达量的影响,以及NtCycB2对外源激素茉莉酮酸甲酯(MeJA)的应答模式;NtCycB2-OE株系喷施不同浓度的MeJA,对其进行叶面腺毛形态观察和密度统计。【结果】(1)植物内源性激素检测结果表明敲除NtCycB2基因后烟叶JA含量显著增加,过表达NtCycB2基因后烟叶JA含量显著降低,其他内源激素的含量未发生显著变化。(2)NtCycB2基因影响JA合成、代谢和信号转导相关基因MFP、JMT和NtJAZ-1、-8、-15表达。(3)NtCycB2转录水平受外源激素MeJA的抑制。(4)MeJA促进NtCycB2-OE植株叶面长柄腺毛的发生。【结论】部分JA信号通路相关基因的表达受NtCycB2的影响,同时JA信号可以抑制NtCycB2的表达,进而促进长柄腺毛的发生。[Objective]This study aims to explore the possible molecular mechanism of NtCycB2 in regulating glandular trichomes generation in tobacco.[Methods]Enzyme linked immunosorbent assay(ELISA)was used to determine the endogenous hormones in leaves of K326,NtCycB2-M and NtCycB2-OE.The effects of NtCycB2 on the expression of genes related to JA signal pathways as well as the response pattern of NtCycB2 to exogenous hormone MeJA were analyzed by qPCR.NtCycB2-OE strain was sprayed with MeJA at different concentrations,and the morphology and density of leaf glandular trichomes were observed.[Results](1)Comparison of plant endogenous hormone contents in K326,NtCycB2-M and NtCycB2-OE showed that the JA content increased significantly in NtCycB2-M,while decreased significantly in NtCycB2-OE,and the contents of other endogenous hormones did not significantly change.(2)NtCycB2 gene affected the gene expressions related in JA synthesis,metabolism and signal transduction,such as MFP JMT and NtJAZ-1,-8,-15.(3)NtCycB2 transcription levels were inhibited by MeJA.(4)MeJA promoted the occurrence of long stalked glandular trichomes in NtCycB2-OE plants.[Conclusion]The expression patterns of some JA signaling genes were affected by NtCycB2,and JA signaling can inhibit NtCycB2 expression and promote the occurrence of long stalk glandular trichomes.

关 键 词：NtCycB2 长柄腺毛发生 MEJA NtJAZ 基因表达 

分 类 号：S572[农业科学—烟草工业]