异枝江蓠对温度和光照强度的光合生理响应  

Photophysiological responses of Gracilariopsis bailinae to temperature andlight intensity

作　　者：黄永健 崔建军 陈心怡 郭优优 辛榕 廖佳炜 陈春丽 谢恩义[1] HUANG Yongjian;CUI Jianjun;CHEN Xinyi;GUO Youyou;XIN Rong;LIAO Jiawei;CHEN Chunli;XIE Enyi(College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088

出　　处：《南方水产科学》2023年第4期139-147,共9页South China Fisheries Science

基　　金：国家重点研发计划“蓝色粮仓科技创新”重点专项(2020YFD0901101)。

摘　　要：大型海藻对温度和光照强度的适应存在种属差异。异枝江蓠(Gracilariopsis bailinae)是一种喜高温的大型海藻,为科学指导该藻在海水养殖、海洋生态修复等方面的应用,利用叶绿素荧光技术,结合藻体光合色素含量和生长率变化,探究了异枝江蓠对温度和光照强度的光合生理响应特征。测定了15、20、25、30、35℃和1000、3000、6000、9000 lx条件下,异枝江蓠的特定生长率(SGR)、光合色素含量[叶绿素a(Chl a)、类胡萝卜素(Car)、藻胆蛋白]及叶绿素荧光参数[PSII最大光化学效率(Fv/Fm)、实际光能转化效率(ΦPSII)、电子传递速率(ETR)、光化学淬灭(qP)、非光化学淬灭(NPQ)]的变化。结果显示,温度和光照强度对上述相关指标(除Car和Fv/Fm)的影响具有极显著的交互作用(P<0.01);温度升高显著增加了异枝江蓠的SGR、藻胆蛋白含量以及叶绿素荧光参数值(P<0.05);高光照显著降低了异枝江蓠的光合色素含量以及Fv/Fm、ΦPSII、ETR和qP值,但NPQ和SGR却显著上升(P<0.05)。结果表明,异枝江蓠是一种喜高温的大型海藻,通过增加藻胆蛋白含量可提高其在高温条件下的生存能力,同时,通过增加热耗散和减少光合色素的合成,可实现藻体在高光下的光保护。There are species differences in the adaptation of macroalgae to temperature and light intensity.Gracilariopsis baili-nae is a large alga that likes high temperature.In order to scientifically guide its application in mariculture and marine ecological restoration,we investigated the photosynthetic physiological responses characteristics of G.bailinae to temperature and light in-tensity by using chlorophyll fluorescence technology,combined with changes in photosynthetic pigment content and growth rate of algae.We measured various physiological parameters,including specific growth rate(SGR),photosynthetic pigments content[Chlorophyll a(Chl a),carotenoid(Car),and phycobiliprotein],and chlorophyll fluorescence parameters[Maximum photo-chemical efficiency of PSII(Fv/Fm),actual light conversion efficiency(ΦPSII),electron transfer rate(ETR),photochemical quenching(qP),and non-photochemical quenching(NPQ)]of G.bailinae cultured at five different temperatures(15,20,25,30,35℃)and four different light intensities(1000,3000,6000,9000 lx).Results reveal that temperatures and light intensities had a significant interaction effect on the parameters measured,except for Car and Fv/Fm(P<0.01).The SGR,phycobiliprotein con-tents,and chlorophyll fluorescence parameters of G.bailinae increased significantly as temperature increased(P<0.05),whereas high light intensity treatment led to a significant decrease in photosynthetic pigments content and values of Fv/Fm,ΦPSII,ETR,and qP,but a significant increase in NPQ and SGR(P<0.05).These results indicate that G.bailinae can enhance its survival capa-city under high temperature conditions by increasing the synthesis of phycobilin,and achieve photoprotection under high light conditions by increasing heat dissipation and decreasing photosynthetic pigment synthesis.

关 键 词：异枝江蓠 光合生理 温度 光照强度 叶绿素荧光参数 

分 类 号：S968.434[农业科学—水产养殖]