黔麦1175茎秆抗倒伏机制的转录组测序分析  

Transcriptome Sequencing Revealed Lodging Resistance Mechanism of Stalk in Qianmai 1175

作　　者：王艳丽 隋建枢 陈天青 王伟 何庆才[3] WANG Yanli;SUI Jianshu;CHEN Tianqing;WANG Wei;HE Qingcai(Guizhou Vocational College Of Agriculture,Qingzhen,Guizhou 551400,China;Guizhou Drought Grain Sorghum Research Institute,Guiyang,Guizhou 550006,China;Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006,China)

机构地区：[1]贵州省农业职业学院,贵州清镇551400 [2]贵州省旱粮研究所,贵州贵阳550006 [3]贵州省农业科学院,贵州贵阳550006

出　　处：《麦类作物学报》2023年第8期958-967,共10页Journal of Triticeae Crops

基　　金：贵州省自然科学基金项目([2018]1151)。

摘　　要：为明确抗倒伏小麦品种黔麦1175茎秆形成的关键因素,在扬花期和成熟期分别测定黔麦1175及易倒伏对照品种黔麦18的生长特性、茎秆特性、纤维素和木质素的含量等性状指标,利用转录组测序技术挖掘调控两个品种茎秆形成的差异表达基因,并对其进行GO功能注释和KEGG富集分析。结果表明,在扬花期,黔麦1175的株高、茎秆重心高度和穗长均显著低于黔麦18,但两品种间的穗重以及茎秆、基部1~2节间的长度和干重均无显著差异;在成熟期,黔麦1175的穗长以及茎秆长度、干重和鲜重均显著低于黔麦18,但穗重、基部1~2节间的长度和干重均无显著差异。黔麦1175的茎秆抗折力、纤维素和木质素的含量均显著高于黔麦18。转录组测序结果表明,各样品的有效数据(clean data)均达到13.85 Gb以上,Q30碱基百分比在93.8%以上。对差异表达基因进行GO功能分类,发现差异表达基因主要涉及催化活性、结合、代谢过程、细胞部分、膜部分、细胞过程、细胞器膜、刺激应答、生物调控、转运活性、转录调控活性等通路;KEGG富集分析结果显示,苯丙烷生物合成、DNA复制、光合作用-天线蛋白、植物激素信号转导等通路富集到的差异表达基因较多。In order to clarify the key factors for the stem formation of the lodging resistant wheat cultivar Qianmai 1175,growth characteristics,stem characteristics,conttents of cellulose and lignin and other traits during the anthesis and maturity stages were comprehensively investigated,with Qianmai 18(easy lodging)as the control.Transcriptome sequencing was performed to reveal the mechanism of stem formation,and GO functional annotation and KEGG enrichment analysis were performed.The results showed that the plant height,gravity center height and ear length of Qianmai 1175 were significantly lower than those of Qianmai 18 at flowering stage,while there was no significant differences in the ear weight,stem length and weight,basal 1-2 internode length and weight.At maturity stage,the ear length,stem gravity center height,length and weight of Qianmai 1175 were significantly lower than those of Qianmai 18,but there was no significant differences in ear weight,basal 1-2 internode length and weight.The stem lodging resistance,cellulose and lignin of Qianmai 1175 were significantly higher than those of Qianmai 18.Transcriptome sequencing showed that clean reads of each sample for transcriptome analysis reached above 13.85 Gb,and the percentage of Q30 base was above 93.8%.GO function classification and KEGG pathway enrichment analysis were performed on differentially expressed genes(DEGs).DEGs were mainly distributed in catalytic activity,binding,metabolic process,cell part,membrane part,cell process,organelle membrane,stimulation response,biological regulation,transport activity and transcriptional regulation activity.KEGG pathway contained phenylpropane biosynthesis,DNA replication,photosynthesis antenna protein and plant hormone signal transduction.

关 键 词：黔麦1175 抗倒伏 木质素 纤维素 差异表达基因 

分 类 号：S512.1[农业科学—作物学] S330