肾不藏志型不寐模型大鼠相关组织IL-10、IL-1β的表达差异研究  

Research on Expression of IL-10 and IL-1βin Related Organs of Insomnia Rat Model with Kidney not Storing Will

作　　者：陈旭 张星平 梁瑞宁 王凯凯 梁政亭 闫德祺 王慧 CHEN Xu;ZHANG Xingping;LIANG Ruining;WANG Kaikai;LIANG Zhengting;YAN Deqi;WANG Hui(The Fourth Clinical College of Xinjiang Medical University,Urumqi 830099,China)

机构地区：[1]新疆医科大学第四临床医学院,新疆乌鲁木齐830099

出　　处：《云南中医药大学学报》2023年第4期81-86,共6页Journal of Yunnan University of Chinese Medicine

基　　金：国家自然科学基金项目(82160873,81960837,81560762,81260526);新疆维吾尔自治区“十三五”重点学科(新教函[2022]112号);全国第六、七批名老中医药专家学术经验继承项目(国中医办人教函[2017]29号、[2022]76号)。

摘　　要：目的探讨肾不藏志型不寐模型大鼠与正常组大鼠相关组织(肾、心、脑、肝、脾、肺、大肠、膀胱)中IL-10、IL-1β的表达水平差异。方法将16只SPF级雄性SD大鼠随机分为模型组与正常组,模型组大鼠使用D-半乳糖联合PCPA以构建肾不藏志型不寐模型大鼠,正常组大鼠同期正常饲养,于第46天处死大鼠后取材,免疫组化法检测两组大鼠相关脏器组织中IL-10、IL-1β的表达水平差异。结果(1)与正常组大鼠比较,模型组大鼠觉醒时间增加(P<0.05),NREM(1,2)期、REM期缩短(P<0.05),NREM(3,4)期无明显差异(P>0.05),表明肾不藏志不寐模型大鼠构建成功;(2)与正常组比较,模型组大鼠睡眠潜伏期延长、睡眠时间缩短(P<0.05);(3)模型组大鼠肾、脑、肺、大肠组织中IL-10的表达水平较正常组降低(P<0.05),IL-1β的表达水平较正常组升高(P<0.05),且均以脑组织变化最为显著(P<0.0001),而两组大鼠肝、心、脾、膀胱组织中IL-10、IL-1β的表达水平均未见明显差异(P>0.05)。结论肾不藏志型不寐模型大鼠肾、脑、肺、大肠组织中IL-10表达水平较正常组大鼠降低,而IL-1β的表达水平较正常组大鼠升高;肾不藏志型不寐模型大鼠肾、脑、肺、大肠组织中IL-10与IL-1β表达水平的差异特点可能是肾不藏志型不寐炎症因子的特征性变化之一。Objective To investigate the levels of IL-10 and IL-1βin related organs(kidney,heart,brain,liver,spleen,lung,large intestine,bladder)of the insomnia rat model with kidney not storing will.Methods A total of 16 male SD rats of SPF grade were randomly divided into model group and normal group,the rats of model group underwent subcutaneous injection in the neck back using D-galactose combined with PCPA to construct a insomnia model rat of kidney not storing will,the normal rats were normally raised during the same period,the rats were sacrificed on the 46th day,then were harvested,and the relevant visceral tissues of the rats in the two groups were detected for the differences in the expression level of IL-10 and IL-1βby immunohistochemistry(IHC).Results(1)Compared with the normal rats,the model rats showed increased wakefulness time(P<0.05),NREM(1,2)phase and REM phase shortened(P<0.05),but there was no significant difference in NREM(3,4)phase(P>0.05),indicating that the kidney not storing will insomnia model rats were constructed successfully;(2)Compared with the normal group,the rats in the model group showed longer sleep latency and shorter sleep time(P<0.05);(3)The expression levels of IL-10 in the kidney,brain,lung,and large intestine tissues of the model rats were decreased compared with those in the normal group(P<0.05),and the expression levels of IL-1βwere increased(P<0.05),and the most significant changes were in brain tissue(P<0.0001),there were no significant differences in the expression levels of IL-10 and IL-1βin liver,heart,spleen,and bladder tissues between two groups(P>0.05).Conclusion The expression levels of IL-10 in the kidney,brain,lung,and large intestine tissues of the insomnia rat model with kidney not storing will were decreased compared with those of normal rats,whereas the expression levels of IL-1βwere increased compared with those of normal rats;the differential expression level of IL-10 and IL-1βin kidney,brain,lung,and large intestine tissues of the insomnia rat model with kidn

关 键 词：肾不藏志 失眠 IL-10 IL-1Β 不寐模型大鼠 

分 类 号：R256.23[医药卫生—中医内科学]