Functionalize d 3D-printe d porous titanium scaffold induces in situ vascularized bone regeneration by orchestrating bone microenvironment  

作　　者：Bo Yuan Pin Liu Rui Zhao Xiao Yang Zhanwen Xiao Kai Zhang Xiangdong Zhu Xingdong Zhang 

机构地区：[1]National Engineering Research Center for Biomaterials,Sichuan University,Chengdu 610064,China [2]College of Biomedical Engineering,Sichuan University,Chengdu 610064,China

出　　处：《Journal of Materials Science & Technology》2023年第22期92-105,共14页材料科学技术（英文版）

基　　金：supported by the Sichuan Science and Technology Program (Nos.2019JDTD0008 and 2022YFG0109);the China Postdoctoral Science Foundation (Nos.2021M692316 and 2020TQ0218).

摘　　要：Titanium(Ti)and its alloys have been extensively explored for treating load-bearing bone defects.How-ever,high-stress shielding,weak osteogenic activity,and insufficient vascularization remain key chal-lenges for the long-term clinical outcomes of Ti-based implants.Herein,inspired by structural and func-tional cues of bone regeneration,a silicon-doped nano-hydroxyapatite(nSiHA)/titanium dioxide(TiO_(2))composite coating with a hierarchical micro/nano-network structure is constructed on the surface of a 3D-printed porous Ti scaffold via a combined strategy of acid-alkali(AA)treatment and electrochemi-cal deposition technique,which not only endows the scaffold with excellent osteoinduction ability but can also effectively immobilize and release vascular endothelial growth factor(VEGF).The results of the in vitro cell experiments show that the functionalized Ti scaffold significantly promotes osteogenesis in bone marrow mesenchymal stem cells(BMSCs)and angiogenesis in human umbilical vein endothelial cells(HUVECs)by activating the extracellular signal-regulated protein kinase(ERK)and HIF-1αsignaling pathways.After being implanted into a rat femoral condyle defect model,the functionalized Ti scaffold can induce in situ vascularized bone regeneration by orchestrating the two coupled processes of angio-genesis and osteogenesis.These findings indicate that the functionalized Ti scaffold has great potential in bone tissue regeneration and is a promising candidate for load-bearing bone defect repair.

关 键 词：Porous titanium 3D-printing Hierarchical micro/nano-structure Bone microenvironment VASCULARIZATION Bone regeneration 

分 类 号：TQ427.26[化学工程] R318[医药卫生—生物医学工程]