转基因棉花再生植株育性影响因素研究  

Study of factors affecting the fertility of regenerated transgenic cotton plants

作　　者：罗晓丽 肖娟丽 王志安 刘圆 张安红 吴家和[2] Luo Xiaoli;Xiao Juanli;Wang Zhian;Liu Yuan;Zhang Anhong;Wu Jiahe(Institute of Cotton Research,Shanxi Agricultural University/Shanxi Key Laboratory of Cotton Germplasm Resources Utilization and Molecular Design Breeding,Yuncheng,Shanxi 044000,China;Institute of Microbiology,Chinese Academy of Sciences/State Key Laboratory of Plant Genomics,Beijing 100101,China)

机构地区：[1]山西农业大学棉花研究所/棉花种质资源利用与分子生物学设计育种山西省重点实验室,山西运城044000 [2]中国科学院微生物研究所/植物基因组学国家重点实验室,北京100101

出　　处：《棉花学报》2023年第3期230-238,共9页Cotton Science

基　　金：国家自然基金(31971905);运城市农业科学合作研究院农业科技攻关项目(2021YNGG09)。

摘　　要：【目的】探究影响农杆菌介导棉花体细胞转化体系产生的再生植株育性的影响因素。【方法】从载体大小、目的基因大小、不同阶段组织培养时间等因素对6个载体的转基因再生植株当代(T0)育性影响进行分析。【结果】不同载体/目的基因的转基因植株不育率呈现显著差异,但是转基因植株育性与载体大小、目的基因大小之间没有明显关系。进一步研究发现转基因再生植株不育率与胚性愈伤组织分化-植株再生的培养时间呈显著正相关,分化-植株再生培养时间少于110 d、110~130 d、130~150 d、150~170 d、超过170 d的植株不育率分别为19.6%、45.5%、63.8%、72.3%、94.5%;而与诱导愈伤组织形成-胚性愈伤组织分化的持续培养时间没有相关性。【结论】转基因棉花再生植株育性受到胚性愈伤组织持续培养时间的显著影响,缩短胚性愈伤组织到植株再生的培养时间能够提高转基因再生植株的育性。[Objective]This study aims to explore factors affecting the fertility of cotton transgenic regenerated plants produced by Agrobacterium-mediated transformation system via somatic cell embryogenesis.[Method]In this study,several factors,including vector size,transgene insertion size,and different duration of culturing period were analyzed for evaluating their effects on the fertility of regenerated transgenic plants(T0 generation).[Result]There were significant differences in sterility rate among transgenic plants of 6 different transgene and expression vector size.But there was no obvious correlation between sterility rate and vector/insertion size.Further analysis showed that the sterility of regenerated transgenic cotton plant had significantly positive correlation with the duration of embryogenic callus differentiation to plantlet regeneration.The sterility rate of regenerated plants from the duration of embryogenic callus differentiation to plantlet regeneration of<110 d,110-130 d,130-150 d,150-170 d,and>170 d were 19.6%,45.5%,63.8%,72.3%,and 94.5%,respectively.However,transgenic cotton plant sterility was not correlated with the duration of callus induction to callus embryogenesis.[Conclusion]Through Agrobacterium-mediated cotton genetic transformation,the sterility of cotton regenerated plants was mainly affected by the duration of embryogenic callus differentiation to plantlet regeneration,and accelerating the procedure of embryogenic callus differentiation could increase the fertility of regenerated transgenic plants.

关 键 词：棉花 转基因再生植株 胚性愈伤组织 分化 植株再生 培养时间 不育率 

分 类 号：S562[农业科学—作物学]