超声波辅助醇提法提取银杏叶中多种活性成分  

Ultrasonic-assisted Ethanol Extraction of Multi-active Components from Ginkgo biloba Leaves

作　　者：李济甫 冯扬帆 孟珂渏 王沈燕 刘鑫 马燕 杨洁[1,2] LI Jifu;FENG Yangfan;MENG Keyi;WANG Shenyan;LIU Xin;MA Yan;YANG Jie(College of Nursing and Health Management&College of Life Science and Chemistry,Wuhan Donghu University,Wuhan 430212,China;Innovation Institute for Biomedical Materials,Wuhan Donghu University,Wuhan 430212,China)

机构地区：[1]武汉东湖学院护理与健康管理学院(生命科学与化学学院),湖北武汉430212 [2]武汉东湖学院生物医用材料创新研究所,湖北武汉430212

出　　处：《化学与生物工程》2023年第8期31-34,共4页Chemistry & Bioengineering

基　　金：湖北省自然科学基金项目(2022CFB197),湖北省教育厅科研计划项目(B2021294),湖北省高校优秀中青年科技创新团队项目(T2022048),湖北省大学生创新创业训练计划项目(S202211798024),2022年度“东湖未来之星”大学生科研基金重点项目(4)。

摘　　要：采用超声波辅助醇提法综合提取银杏叶中多种活性成分,通过溶剂萃取法脱脂、去叶绿素,经DM130大孔树脂吸附脱附后,再对银杏多糖、银杏莽草酸、银杏黄酮分阶段逐一分离纯化,并对工艺条件进行了优化。结果表明,在75%乙醇为提取溶剂、超声时间为2.0 h、石油醚为脱脂溶剂的最佳条件下,分别采用0.2%H 2O 2脱色法、石油醚沉淀法、酸碱除杂法对银杏多糖、银杏莽草酸、银杏黄酮进行分离纯化,得到高纯度银杏多糖、银杏莽草酸、银杏黄酮,其纯度分别为85.20%、98.25%、35.72%,产率分别为4.67%、3.80%、0.77%。该方法工艺简单、成本低、资源利用度高,为银杏叶的综合开发利用提供了参考。Firstly,we comprehensively extracted multi-active components from Ginkgo biloba leaves by ultrasonic-assisted ethanol extraction method.Then,through degrease and removing chlorophyll by solvent extraction,adsorption-desorption on DM130 macroporous resin,and separation and purification one by one in stages,we obtained the ginkgo polysaccharides,ginkgo shikimic acid,and ginkgo flavonoids.Moreover,we optimized the process conditions.The results show that,high-purity ginkgo polysaccharides,ginkgo shikimic acid,and ginkgo flavonoids are obtained under the optimal conditions as follows:75%ethanol is adopted as the extraction solvent,the ultrasonic time is 2.0 h,and petroleum ether is adopted as the degreasing solvent;separation and purification of ginkgo polysaccharides,ginkgo shikimic acid,and ginkgo flavonoids by 0.2%H 2O 2 decolorization,petroleum ether precipitation,and acid-base impurity removal,respectively.The purities of the ginkgo polysaccharides,ginkgo shikimic acid,and ginkgo flavonoids are 85.20%,98.25%,and 35.72%,respectively,and the yields are 4.67%,3.80%,and 0.77%,respectively.The method has the advantages of simple process,low cost,and high resource utilization,which can provide a reference for the comprehensive development and utilization of Ginkgo biloba leaves.

关 键 词：银杏叶 多糖 莽草酸 黄酮 综合提取 超声波 

分 类 号：TQ461[化学工程—制药化工] R284[医药卫生—中药学]