The Jasmine(Jasminum sambac)Genome Provides Insight into the Biosynthesis of Flower Fragrances and Jasmonates  

作　　者：Gang Chen Salma Mostafa Zhaogeng Lu Ran Du Jiawen Cui Yun Wang Qinggang Liao Jinkai Lu Xinyu Mao Bang Chang Quan Gan Li Wang Zhichao Jia Xiulian Yang Yingfang Zhu Jianbin Yan Biao Jin 

机构地区：[1]College of Horticulture and Plant Protection,Yangzhou University,Yangzhou 225009,China [2]College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009,China [3]Department of Floriculture,Faculty of Agriculture,Alexandria University,Alexandria 21526,Egypt [4]Key Laboratory of Plant Functional Genomics of the Ministry of Education,Yangzhou University,Yangzhou 225009,China [5]Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Key Laboratory of Synthetic Biology,Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China [6]College of Landscape Architecture,Nanjing Forestry University,Nanjing 210037,China [7]Institute of Plant Stress Biology,State Key Laboratory of Cotton Biology,Department of Biology,Henan University,Kaifeng 475001,China

出　　处：《Genomics, Proteomics & Bioinformatics》2023年第1期127-149,共23页基因组蛋白质组与生物信息学报（英文版）

基　　金：We thank Novogene for genome sequencing and assembly.We thank Dr.Feng Cheng for his comments on our manuscript.This work was supported by the Elite Young Scientists Program of Chinese Academy of Agricultural Sciences(CAAS),the Agricultural Science and Technology Innovation Program in China and self-raised funds.

摘　　要：Jasminum sambac(jasmine flower),a world-renowned plant appreciated for its exceptional flower fragrance,is of cultural and economic importance.However,the genetic basis of its fragrance is largely unknown.Here,we present the first de novo genome assembly of J.sambac with 550.12 Mb(scaffold N50=40.10 Mb)assembled into 13 pseudochromosomes.Terpene synthase(TPS)genes associated with flower fragrance are considerably amplified in the form of gene clusters through tandem duplications in the genome.Gene clusters within the salicylic acid/benzoic acid/theobromine(SABATH)and benzylalcohol O-acetyltransferase/anthocyanin O-hydroxycinnamoyltransferases/anthranilate N-hydroxycinnamoyl/benzoyltransferase/deacetylvindoline 4-O-acetyltransferase(BAHD)superfamilies were identified to be related to the biosynthesis of phenylpropanoid/benzenoid compounds.Several key genes involved in jasmonate biosynthesis were duplicated,causing an increase in copy numbers.In addition,multi-omics analyses identified various aromatic compounds and many genes involved in fragrance biosynthesis pathways.Furthermore,the roles of JsTPS3 in b-ocimene biosynthesis,as well as JsAOC1 and JsAOS in jasmonic acid biosynthesis,were functionally validated.The genome assembled in this study for J.sambac offers a basic genetic resource for studying floral scent and jasmonate biosynthesis,and provides a foundation for functional genomic research and variety improvements in Jasminum.

关 键 词：Jasmine flower Jasminum sambac GENOME Jasmonate Flower fragrance 

分 类 号：S685.16[农业科学—观赏园艺]