LCHAD基因表达及甲基化与病理妊娠状态相关性研究  

Study on the correlation between the expression and methylation of LCHAD gene and pathological pregnancy state

作　　者：季金龙[1] 陈丽平[1] 韩云[1] 刘颖蕾[1] 汤卫春 尤珺 JI Jin-long;CHEN Li-ping;HAN Yun;LIU Ying-lei;TANG Wei-chun;YOU Jun(Department of Obstetrics and Gynecology,The Second Affiliated Hospital of Nantong University,Nantong Jiangsu 226000,China)

机构地区：[1]南通大学第二附属医院妇产科,江苏南通226000

出　　处：《蚌埠医学院学报》2023年第7期917-921,共5页Journal of Bengbu Medical College

基　　金：江苏省妇幼健康科研项目(F201929);江苏省南通市卫生和计划生育委员会科研项目青年课题(QA2019005)。

摘　　要：目的:探讨长链3-羟酰基辅酶A脱氢酶(LCHAD)基因表达及甲基化与病理妊娠状态的相关性。方法:选取早发重度子痫前期病人18例(A组)、溶血、肝酶升高和血小板减少(HELLP)综合征15例(B组)、抗磷脂综合征17例(C组),同时选取正常妊娠女性20名作为对照组。比较各组一般资料、LCHAD基因启动子区甲基化及LCHAD mRNA表达水平,分析LCHAD基因启动子区甲基化水平与mRNA表达相关性。结果:A、B组分娩孕周低于对照组(P<0.05),C组分娩孕周高于B组(P<0.05);A、B组收缩压、舒张压及24 h尿蛋白水平均高于对照组(P<0.05);B组丙氨酸氨基转移酶和血肌酐水平均高于对照组(P<0.05)。A组LCHAD基因-899、-853、-615、-984、-774、-727及-579位点甲基化水平高于对照组,-853位点甲基化水平高于B组,-899、-853及-615位点甲基化水平高于C组(P<0.05);B组-899、-853、-774及-615位点甲基化水平高于对照组,-899、-853及-615位点甲基化水平高于C组(P<0.05)。A组、B组、C组LCHAD mRNA表达水平均低于对照组(P<0.05)。A组LCHAD基因-899、-853、-727、-615及-579位点甲基化水平与mRNA表达水平均呈负相关关系(P<0.05);B组LCHAD基因-899、-853及-615位点甲基化水平与mRNA表达水平均呈负相关关系(P<0.05);C组和对照组LCHAD基因全部位点甲基化水平与mRNA表达水平均无明显相关关系(P>0.05)。结论:合并早发重度子痫前期和HELLP综合征孕妇LCHAD基因甲基化修饰处于高水平,且与mRNA表达密切相关。Objective:To investigate the correlation between the expression and methylation of long-chain 3-hydroxyacyl-CoA dehydrogenase(LCHAD)gene and pathological pregnancy state.Methods:Eighteen cases with early-onset severe preeclampsia were selected as group A,15 cases with hemolysis,elevated liver enzymes and low platelets(HELLP)syndrome as group B,17 cases with antiphospholipid syndrome as group C,and 20 normal pregnant women as the control group.The general data,methylation of the promoter region of LCHAD gene and the expression level of LCHAD mRNA in each group were compared,and the correlation between the methylation level of promoter region of LCHAD gene and mRNA expression was analyzed.Results:The gestational age in group A and group B was lower than that in control group(P<0.05),which in group C was higher than that in group B(P<0.05);the systolic blood pressure,diastolic blood pressure and 24 h urine protein level in group A and group B were higher than those in control group(P<0.05);the levels of alanine aminotransferase and serum creatinine in group B were higher than those in control group(P<0.05).The methylation level at-899,-853,-615,-984,-774,-727 and-579 site of LCHAD gene in group A was higher than that in control group,which at-853 site was higher than that in group B,and which at-899,-853 and-615 site higher than that in group C(P<0.05).The methylation level at-899,-853,-774 and-615 site of LCHAD gene in group B was higher than that in control group,which at-899,-853band-615 site was higher than that in group C(P<0.05).The expression of LCHAD mRNA in group A,group B and group C was lower than that in control group(P<0.05).The methylation level at-899,-853,-727,-615 and-579 site of LCHAD gene in group A was negatively correlated with the mRNA expression level(P<0.05);the methylation level at-899,-853 and-615 site of LCHAD gene in group B was negatively correlated with the mRNA expression level(P<0.05);the methylation level at all sites of LCHAD gene in group C had no significant correlation with the m

关 键 词：妊娠 长链3-羟酰基辅酶A脱氢酶 甲基化 

分 类 号：R714.2[医药卫生—妇产科学]