旋覆代赭汤抑制炎症因子分泌缓解过敏性哮喘的机制  

Mechanism of Xuanfu Daizhetang in Alleviating Allergic Asthma by Inhibiting Secretion of Inflammatory Factors

作　　者：柴南南 李晓艺 刘明秀 郭晶 濮兴娜 何红平 李艳平 CHAI Nannan;LI Xiaoyi;LIU Mingxiu;GUO Jing;PU Xingna;HE Hongping;LI Yanping(Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]云南中医药大学,昆明650000

出　　处：《中国实验方剂学杂志》2023年第17期26-33,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：云南省高层次人才培养支持计划“青年拔尖人才”专项(YNWR-QNBJ-2020-249);云南省应用基础研究计划项目中医联合专项[2018FF001(-029)]。

摘　　要：目的:研究旋覆代赭汤对卵清蛋白(OVA)诱导的小鼠过敏性哮喘模型的调节作用。方法:60只雌性BALB/c小鼠(6~8周,SPF);随机分选10只作为正常组给予生理盐水0.2 mL,其余组分别腹腔注射5 g·L^(-1)的氢氧化铝Al(OH)3与1 g·L^(-1)的OVA。分为正常组(10 mL·kg^(-1)生理盐水)、鸡OVA模型组(10 mL·kg^(-1)生理盐水)、地塞米松组(DEX,1 mg·kg^(-1))、旋覆代赭汤低、中、高剂量组(7.065、14.13、28.26 g·kg^(-1))。建立OVA诱导的小鼠哮喘模型。运用苏木素-伊红(HE)和过碘酸雪夫(PAS)染色法,观察支气管组织病理学改变;酶联免疫吸附测定法(ELISA)检测肺泡灌洗液中免疫球蛋白E(IgE)、白细胞介素-4(IL-4)、白细胞介素-5(IL-5)、白细胞介素-13(IL-13)、白细胞介素-17A(IL-17A)和γ干扰素(IFN-γ)的水平;蛋白免疫印迹法(Western blot)检测肺组织中细胞外调节蛋白激酶(ERK)、p38丝裂原活化蛋白激酶(MAPK)和c-Jun氨基末端激酶(JNK)的蛋白的磷酸化。结果:与正常组比较,模型组增加了小鼠肺泡内炎性细胞浸润,升高了肺泡灌洗液中IL-4、IL-5、IL-13、IL-17A、IFN-γ和血清中IgE含量(P<0.05,P<0.01),促进了MAPK信号通路相关蛋白的磷酸化;与模型组比较,旋覆代赭汤低、中、高剂量组减少了小鼠肺泡内炎性细胞浸润,明显降低了肺泡灌洗液中IL-4、IL-5、IL-13、IL-17A、IFN-γ和血清中IgE含量(P<0.05,P<0.01),抑制了MAPK信号通路相关蛋白的磷酸化。结论:本研究结果说明旋覆代赭汤具有潜在的抗过敏性哮喘的活性,为其以后应用于临床提供了一部分理论依据。Objective:To investigate the regulatory effects of Xuanfu Daizhetang on a mouse model of allergic asthma induced by ovalbumin(OVA).Method:Sixty female BALB/c mice(6-8 weeks old,SPF)were randomly divided into groups.Ten mice were assigned to the normal group and given 0.2 mL of saline,while the remaining groups received intraperitoneal injections of Al(OH)3 at 5 g·L^(-1) and OVA at 1 g·L^(-1).The mice were divided into normal group(10 mL·kg^(-1) saline),OVA model group(10 mL·kg^(-1) saline),dexamethasone group(OVA+DEX,1 mg·kg^(-1)),OVA+low-dose Xuanfu Daizhetang group(OVA+XL,7.065 g·kg^(-1)),OVA+medium-dose Xuanfu Daizhetang group(OVA+XM,14.13 g·kg^(-1)),and OVA+high-dose Xuanfu Daizhetang group(OVA+XH,28.26 g·kg^(-1)).An OVA-induced asthma model was established in mice.Hematoxylin-eosin(HE)and periodic acid-Schiff(PAS)staining methods were used to observe bronchial tissue pathological changes.Enzyme-linked immunosorbent assay(ELISA)was performed to measure the levels of immunoglobulin E(IgE),interleukin-4(IL-4),IL-5,IL-13,IL-17A,andγinterferon(IFN-γ)in bronchoalveolar lavage fluid.Western blot was used to detect the phosphorylation of extracellular signalregulated kinase(ERK),p38 mitogen-activated protein kinase(MAPK),and c-Jun N-terminal kinase(JNK)proteins in lung tissue.Result:Compared with the normal group,the OVA model group showed increased inflammatory cell infiltration in mouse alveoli,elevated levels of IL-4,IL-5,IL-13,IL-17A,IFN-γin bronchoalveolar lavage fluid,and IgE in serum(P<0.05,P<0.01),and promoted phosphorylation of MAPK signaling pathway-related proteins.Compared with the model group,the OVA+XL,OVA+XM,and OVA+XH groups showed reduced inflammatory cell infiltration in mouse alveoli,decreased levels of IL-4,IL-5,IL-13,IL-17A,IFN-γin bronchoalveolar lavage fluid,and IgE in serum(P<0.05,P<0.01),and inhibited phosphorylation of MAPK signaling pathway-related proteins.Conclusion:The results of this study suggest that Xuanfu Daizhetang has potential anti-allergic asthma activity,providing

关 键 词：旋覆代赭汤 过敏性哮喘 气道炎症 丝裂原活化蛋白激酶(MAPK)信号通路 

分 类 号：R2-0[医药卫生—中医学] R22R285.5R289R33