丹参酮ⅡA磺酸钠对TGF-β1诱导的心房纤维化效应的影响  

Effects of sodium tanshinoneⅡA sulfonate on TGF-β1-induced atrial fibrosis

作　　者：杨乐[1] 刘刚 郝洪真 邹晓静[3] YANG Le;LIU Gang;HAO Hongzhen;ZOU Xiaojing(Department of Emergency Medicine,Department of Critical Care Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei Wuhan 430030,China;Department of Pharmacology,Tongji Medical College of Huazhong University of Science and Technology,Hubei Wuhan 430030,China;Department of Critical Care Medicine,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei Wuhan 430022,China)

机构地区：[1]华中科技大学同济医学院附属同济医院急诊科/重症医学科,湖北武汉430030 [2]华中科技大学同济医学院药理学系,湖北武汉430030 [3]华中科技大学同济医学院附属协和医院重症医学科,湖北武汉430022

出　　处：《中国医院药学杂志》2023年第14期1550-1553,共4页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金项目(编号:81102691,30901405);湖北省自然科学基金项目(编号:2011CDB538);湖北省卫健委科研面上项目(编号:WJ2023M008)。

摘　　要：目的:观察丹参酮ⅡA磺酸钠(sodium tanshinoneⅡA sulfonate,STS)对转换生长因子-β1(TGF-β1)诱导的心房纤维化效应的影响。方法:培养新生乳鼠心房成纤维细胞。MTT比色法测定心房成纤维细胞增殖率,[3H]脯氨酸掺入测定心房成纤维细胞合成胶原速率。共聚焦荧光显微镜及Western blot检测Ⅰ/Ⅲ型胶原表达、Smad2磷酸化及核转移。结果:TGF-β1处理24 h后,心房成纤维细胞增殖明显增强,同时Ⅰ/Ⅲ型胶原表达及Smad2磷酸化/核转移亦明显增强。丹参酮ⅡA磺酸钠各浓度组以上效应均减弱。结论:丹参酮-ⅡIA磺酸钠能明显减轻TGF-β1诱导的心房纤维化效应,机制与调控Smad2磷酸化及核转移相关。OBJECTIVE To investigate the functions of sodium tanshinoneⅡA sulfonate(STS)on TGF-β1-induced the activation of TGF-β1 and syntheses collagen protein in atrial fibroblasts(AFs).METHODS In the primary culture of neonatal rat AFs,as indexes of myocardial fibrosis,the cell proliferation was determined by MTT.Collagen synthesis rate was measured by[3H]proline incorporation.The level ofⅠ/Ⅲcollagen protein was determined by Western blot.Smad2 phosphorylation/nuclear translocation was measured by fluorescent confocal microscopy and Western blot.RESULTS TGF-β1 could increase the level ofⅠ/Ⅲcollagen protein,cell proliferation.Furthermore,TGF-β1 could increase Smad2 phosphorylation/nuclear translocation.After treatment of STS,these indexes were obviously downregulated.CONCLUSION STS can decrease TGF-β1-induced atrial fibrosis in atrial fibroblasts,through inhibiting Smad2 phosphorylation/nuclear translocation.

关 键 词：丹参酮-ⅡA磺酸钠 心房纤维化 转化生长因子-Β1 

分 类 号：R285.5[医药卫生—中药学]