固氮根瘤菌与海南主栽菜用大豆的共生匹配性和促生效应  

Symbiotic Compatibility and Growth Promotion Effect of Nitrogen Fixing Rhizobia on Hainan Vegetable-type Soybean

作　　者：朱佳豪 焦健 蒲怡 林清喜 林杉[1,2] ZHU Jiahao;JIAO Jian;PU Yi;LIN Qingxi;LIN Shan(Sanya Institute of China Agricultural University,Sanya 572024,China;College of Resources and Environmental Sciences,China Agricultural University,Beijing 100193,China;College of Biological Sciences,China Agricultural University,Beijing 100193,China;Ledong Lyusheng Agricultural Development Co.,Ltd,Sanya 572500,China)

机构地区：[1]中国农业大学三亚研究院,海南三亚572024 [2]中国农业大学资源与环境学院,北京100193 [3]中国农业大学生物学院,北京100193 [4]乐东绿盛农业开发有限公司,海南三亚572500

出　　处：《大豆科学》2023年第4期441-450,共10页Soybean Science

基　　金：三亚崖州湾科技城管理局资助项目(SYND-2021-10);中国烟草总公司云南省烟草公司重点科技计划项目(202153000-0241032)。

摘　　要：为筛选与海南主栽菜用大豆品种“六四”、“翠绿宝”和“铁豆”匹配性高且固氮能力强的大豆根瘤菌,本研究选择了8株分离自不同生态区的根瘤菌,采用低氮蛭石基质栽培方法进行培养和筛选,测定了茎叶干重、叶片SPAD值、根干重、根瘤干重、根瘤数量等指标,并对其进行结构方程模型分析和相关性分析,以评价不同根瘤菌对菜用大豆生长的影响。结果表明:(1)不同菜用大豆品种接种不同根瘤菌促生效果差异显著,接种CCBAU 25435对菜用大豆茎叶干重、叶片SAPD值等生长指标的提高效果显著,较不接种根瘤菌增加80%和112%,与国际上广泛接种的高效固氮根瘤菌USDA 110相比无明显差异甚至略优;(2)叶片SPAD值、根干重、瘤干重对茎叶干重的形成有显著的正直接效应,其中叶片SPAD值的影响权重最高,根瘤数则通过前三者间接促进了茎叶干重的形成;(3)中效和高效结瘤固氮根瘤菌的根瘤数和瘤干重显著高于无效菌,而高效菌的单位瘤干重显著低于中效菌。本研究筛选得到的高效固氮根瘤菌为海南地区根瘤菌剂的开发应用提供了优质根瘤菌资源。In order to screen the soybean rhizobium with high compatibility and strong nitrogen fixing ability with the main vegetable soybean varieties Liusi,Cuilyubao and Tiedou in Hainan Province.In this study,8 strains of rhizobium isolated from different ecological regions were selected for culture and screening using low nitrogen vermiculite substrate cultivation method.Stem and leaf dry weight,leaf SPAD value,root dry weight,root nodule dry weight,root nodule number and other indexes were determined,and structural equation model analysis and correlation analysis were conducted to evaluate the effects of different rhizobia on the growth of vegetable soybean.The results showed as follows:(1)There were significant differences in the growth promotion effects of different varieties of vegetable soybean inoculated with different rhizobia.The effects of inoculation with CCBAU 25435 on the stem and leaf dry weight,leaf SAPD value and other growth indexes of vegetable soybean were significantly increased by 80%and 112%compared with those without inoculation with rhizobia.There was no significant difference or even slightly better than the highly effective nitrogen-fixing rhizobium USDA 110 widely inoculated internationally.(2)Leaf SPAD value,root dry weight and nodules dry weight had significant positive and direct effects on the formation of shoot dry weight,among which leaf SPAD value had the highest influence weight,and nodules number indirectly promoted the formation of shoot dry weight through the first three factors.(3)The number of nodules and dry weight of nitrogen-fixing rhizobia of medium-effective and high-efficient nodules were significantly higher than those of ineffective bacteria,while the dry weight of unit nodules of efficient bacteria was significantly lower than that of medium-effective bacteria.This study revealed the relationship between root nodule traits and plant growth.The root nodule biomass was not strictly positive correlated with the nitrogen-fixation promoting effect,and the nodule number could b

关 键 词：根瘤菌筛选 共生匹配性 固氮能力 结瘤性状 结构方程模型 

分 类 号：S643.7[农业科学—蔬菜学]