60份大豆种质资源苗期耐盐性鉴定评价  

Evaluation of Salt Tolerance in 60 Soybean Germplasm Resources at Seedling Stage

作　　者：韩岱 时晓磊 丁孙磊 张金波[2] 严勇亮[2] HAN Dai;SHI Xiaolei;DING Sunlei;ZHANG Jinbo;YAN Yongliang(Agricultural College of Xinjiang Agricultural University/Key Laboratory of Agricultural Biotechnology,Urumqi 830052,China;Institute of Crop Germplasm Resources,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)

机构地区：[1]新疆农业大学农学院/农业生物技术重点实验室,新疆乌鲁木齐830052 [2]新疆农业科学院农作物品种资源研究所,新疆乌鲁木齐830091

出　　处：《大豆科学》2023年第4期494-505,共12页Soybean Science

基　　金：中央引导地方科技发展专项资金项目(ZYYD2022C02);新疆维吾尔自治区重大科技专项(2022A03004);新疆维吾尔自治区区域协同创新专项(2020E01005);国家现代农业产业技术体系(CARS-04-CES31)。

摘　　要：为挖掘大豆种质资源中的耐盐种质资源,筛选出耐盐性较强的种质资源,为大豆耐盐性品种的选育提供优异亲本,本研究选用来源于黑龙江、吉林、日本等地的大豆种质资源60份,采用1.500%NaCl溶液模拟盐胁迫环境进行苗期耐盐性鉴定评价,测定叶长、叶宽、叶柄长度、株高、茎秆直径、茎节数、分枝数、植株鲜重、植株干重等9个性状指标,应用隶属函数、主成分分析、聚类分析等方法对大豆苗期耐盐性进行综合评价。结果表明:盐胁迫下各指标测量值与对照相比较均明显下降,且各指标之间呈现极显著或显著相关关系。利用隶属函数法进行耐盐性综合评价,发现不同大豆品种间的耐盐性表现出较大的差异,60份大豆中包括耐盐品种12份,中等耐盐品种24份,盐敏感品种16份,不耐盐品种8份。9个性状可综合为3个主成分,可解释67.430%的表型变异,分别代表叶片因子、伸长因子和生物量因子;60份材料按耐盐性强弱可分为4类,第一类为耐盐品种(12份),第二类为中等耐盐品种(24份),第三类为敏感品种(16份),第四类为不耐盐品种(8份)。东农56、佳试D、黑农63耐盐性较强,叶长、分枝数、地上部分干重均可作为大豆耐盐筛选与评价的重要指标。In order to explore salt-tolerant germplasm resources in soybean germplasm resources,select the germplasm resources with strong salt tolerance,and provide excellent parents for the breeding of soybean salt-tolerant varieties,in this study,60 soybean germplasm resources from Heilongjiang,Jilin and Japan were selected.Salt tolerance at seedling stage was evaluated by using 1.500%NaCl solution to simulate salt stress environment.Nine traits including leaf length,leaf width,leaf stalk length,plant height,stem diameter,number of stem nodes,number of branches,plant fresh weight and plant dry weight were determined.Salt tolerance of soybean seedling was evaluated by membership function,principal component analysis and cluster analysis.The results showed that the measured values of each index decreased significantly under salt stress,and the correlation between each index was extremely significant or significant.Salt tolerance was evaluated by membership function method,and it was found that salt tolerance of different soybean varieties showed great differences.Among the 60 soybean varieties,12 were salt tolerant,24 were medium salt tolerant,16 were salt sensitive and 8 were salt intolerant.The nine characters could be integrated into three principal components,representing leaf factor,elongation factor and biomass factor,which could explain 67.430%of phenotypic variation.According to the strength of salt tolerance,the 60 materials could be divided into 4 categories.The first category was salt-tolerant varieties(12 copies),the second category was medium salt-tolerant varieties(24 copies),the third category was sensitive varieties(16 copies),and the fourth category was intolerant varieties(8 copies).Dongnong 56,Jiashi D and Heinong 63 showed strong salt tolerance.The leaf length,branches number and aboveground dry weight could be used as important indexes for screening and evaluation of salt tolerance of soybean.

关 键 词：大豆 苗期 耐盐性 隶属函数分析 

分 类 号：S565.1[农业科学—作物学]