2-脱氧葡萄糖通过调控山桃SnRK1促进根毛的发生和生长  

2-Deoxyglucose promotes root hair generation and growth by regulating PdSnRK1

作　　者：于文英 吴雪莲 梁家慧 张淑辉 肖元松[1] 彭福田[1] YU Wenying;WU Xuelian;LIANG Jiahui;ZHANG Shuhui;XIAO Yuansong;PENG Futian(State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University Taian Shandona 271018 China)

机构地区：[1]山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安271018

出　　处：《植物生理学报》2023年第7期1291-1299,共9页Plant Physiology Journal

基　　金：国家现代桃产业技术体系(CARS-30-2-02)。

摘　　要：本试验以山桃实生苗为试材,研究了不同浓度2-脱氧葡萄糖(2-DG)对蔗糖非发酵相关蛋白激酶-1(SnRK1)活性及其编码基因PdSnRK1α表达量的影响,并进一步分析其对根毛生长的作用。试验结果表明:随着2-DG浓度的升高,PdSnRK1α的表达水平及SnRK1酶活性呈现先上升后下降的趋势。当2-DG的浓度为400 mg·L^(-1)时,根中PdSnRK1α基因表达量及SnRK1酶活性最高,根毛密度显著增加,并且这种促进作用会被SnRK1蛋白激酶抑制剂海藻糖(Tre)削弱。拟南芥中超表达PdSnRK1α可以显著增加根毛数量与长度;与野生型相比,根毛生长发育相关基因表达量上调。综上表明,2-DG能够上调PdSnRK1α表达量,提高SnRK1酶活性进而促进桃根毛的发生和生长。In this study,the seedlings of Prunus davidiana were used as the test material,the effects of different concentrations of 2-deoxyglucose(2-DG)on the activity of sucrose non-fermenting-related kinase 1(SnRK1)and the expression of its encoding gene PdSnRK1a were studied.The effects of 2-DG on root hair growth were further analyzed.The results showed that with the increase of 2-DG concentration,the expression level of PdSn-RK1a and the activity of SnRK1 firstly increased and then decreased.When the concentration of 2-DG was 400 mg·L^(-1),the expression of PdSnRK1a and the activity of SnRK1 in root were the highest,and the density of root hair was significantly increased.The promoting effect of 2-DG was weakened by the SnRK1 protein kinase inhibitor trehalose(Tre).Overexpression of PdSnRK1a in Arabidopsis thaliana increased the number and length of root hairs.Compared with the wild type,the expressions of genes related to root hair growth and development were up-regulated.In conclusion,2-DG can up-regulate the expression of PdSnRK1a,improve the activity of SnRK1,and promote the occurrence and growth of root hair in peach.

关 键 词：山桃 SnRK1 2-脱氧葡萄糖 海藻糖 根毛 

分 类 号：Q945[生物学—植物学]