苹果Nramp基因组鉴定及其对不同重金属离子的反应  

Genome identification of apple Nramp and its response to different heavymetalions

作　　者：石宇佳 李佳琦 宋建飞 严俊红 朱晓悦 张晓健 张玮玮[1] 杨洪强[1] SHI Yujia;LI Jiaqi;SONG Jianfei;YAN Junhong;ZHU Xiaoyue;ZHANG Xiaojian;ZHANG Weiwei;YANG Hongqiang(State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Taian,Shandong 21018,China)

机构地区：[1]山东农业大学园艺科学与工程学院作物生物学国家重点实验室,山东泰安271018

出　　处：《植物生理学报》2023年第7期1361-1369,共9页Plant Physiology Journal

基　　金：国家自然科学基金(32172517);国家重点研发计划(2019YFD100103)。

摘　　要：天然抗性相关巨噬蛋白(natural resistance-associated macrophage protein)是一类能够转运Fe^(2+)和Cd^(2+)等二价金属离子的蛋白。本文从苹果(Malus pumila)基因组内分离鉴定出8个Nramp同源基因,根据其进化关系、编码蛋白的氨基酸数及理化性质的相似性分为3组;WoLF PSORT预测它们所编码的蛋白全部定位于质膜;PlantCARE显示8个MpNramps的启动子序列中含有多个激素响应及非生物胁迫响应元件;表达谱分析表明它们在叶片中的表达量因苹果品种不同而有较大差异。在Fe^(2+)、Mn^(2+)、Zn^(2+)、Cu^(2+)和Cd^(2+)五种重金属离子处理下,8个MpNramps在‘嘎啦’苹果组培苗叶片的表达水平差异显著,其中,MpNramp4对Fe^(2+)和Mn^(2+)反应较敏感,MpNramp1和MpNramp6对Mn^(2+)和Zn^(2+)较敏感,MpNramp2和MpNramp3对Fe^(2+)、Mn^(2+)和Zn^(2+)处理都比较敏感。8个MpNramps在Cu^(2+)和Cd^(2+)处理下都明显上调表达,对毒性重金属均呈现出较高的敏感性。Natural resistance-associated macrophage proteins are a class of proteins capable of transporting divalent metal ions,such as Fe2*and Cd2.Eight Nramp homologous genes were isolated and identified from apple(Malus pumila)genome which were divided into three groups based on their evolutionary relationship,amino acid numbers and physicochemical properties.WoLF PsORT predicted that all the proteins were located in the plasma membrane.PlantCARE showed that their promoter sequences contained multiple hormone response elements and abiotic stress response elements.Analysis of expression profiles showed that the levels of their expression in apple leaves varied considerably with different varieties.When treated with Fe^(2+),Mn^(2+),Zn^(2+),Cu^(2+)and Cd^(2+),the expression levels of eight MpNramps were significantly different in the leaves of'Gala'apple(M.pumila'Gala')tissue culture seedlings.Among them,MpNramp4 was sensitive to Fe^(2+)and Mn^(2+),MpNramp1 and MpNramp6 were sensitive to Mn^(2+)and Zn^(2+),while Mp Nramp2 and MpNramp3 were sensitive to Fe^(2+),Mn^(2+)and Zn^(2+).All eight MpNramps were up-regulated under Cu^(2+)and Cd^(2+)treatment,indicating a high sensitivity to toxic heavy metals.

关 键 词：苹果 Nramp基因 重金属 基因表达 

分 类 号：X503.231[环境科学与工程—环境工程] X171.5[农业科学—果树学] S661.1[农业科学—园艺学]