油茶籽成熟过程中油脂代谢的蛋白质组学分析  

Proteomic analysis of lipid metabolism in Camellia oleifera seeds during maturation

作　　者：沈军 邱智敏 余玉云 李志真[3] 陈伟[4] 陈世品[1] SHEN Jun;QIU Zhimin;YU Yuyun;LI Zhizhen;CHEN Wei;CHEN Shipin(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujan Academy of Forestry Sciences,Fuzhou 350002,China;College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学林学院,福州350002 [2]福建农林大学园艺学院,福州350002 [3]福建省林业科学研究院,福州350002 [4]福建农林大学生命科学学院,福州350002

出　　处：《植物生理学报》2023年第7期1398-1406,共9页Plant Physiology Journal

基　　金：福建省种业创新与产业化工程(2021~2025年)-油茶种业创新与产业化开发(ZYCX-LY-202103)。

摘　　要：以普通油茶(Camellia oleifera)优良无性系‘闽43’为实验材料,进行生理试验和酶活性检测,采用定量蛋白质组学技术对油茶成熟过程中3个时期的籽仁进行分析,探讨油茶籽油脂代谢的调控机制。研究发现,共有284个蛋白差异表达,依据功能分为17类,其中脂质代谢与运输相关蛋白多数呈现下调表达。硬脂酰-ACP去饱和酶(SAD)和胆碱激酶(CK)蛋白表达量的下调,与不饱和脂肪酸生成速率的减缓有关;在油茶果实成熟过程中的第二个阶段,脂肪酶(LPS)活性的下调造成游离脂肪酸的产生减少。由于长链脂酰辅酶A合成酶(LACS)蛋白表达量的下调,亚油酸和亚麻酸以游离脂肪酸的形式存在较长时间,促使脂氧合酶(LOX)的活性上升,导致该时期亚油酸和亚麻酸含量的下降。研究结果表明,油茶籽成熟过程中,不饱和脂肪酸含量的调控与油脂代谢相关蛋白表达模式和酶活性的变化密切相关。研究结果为选育高品质的油茶品种提供理论依据。In order to explore the regulation mechanism of oil metabolism of Camellia oleifera,a superior clone of common camellia'Min 43'was used as experimental material to analyze the seed kernel of three stages during its maturation period by using quantitative proteomics technology through physiological test and enzyme activity detection.The results show that a total of 284 proteins were differentially expressed and divided into 17 categories according to their functions.Most of the proteins related to lipid metabolism and transportation were down-regulated.The down-regulation of the expression levels of stearyl-ACP desaturase(SAD)and choline kinase(CK)was related to the decrease of the rate of unsaturated fatty acid production.The down-regulation of lipase(LPS)activity during P2 reduced the production of free fatty acids.Due to the down-regulation of long-chain fatty acyl-CoA synthase(LACS)protein expression,both linoleic acid and linolenic acid would exist in the form of free fatty acids for a long time,which led to the increase of lipid oxygenase(Lox)activity and the decrease of linoleic acid content in this period.The results show that the regulation of unsaturated fatty acid content was closely related to the changes of lipid metabolization related protein expression patterns and enzyme activities during the maturation of C.oleifera seed.It provides theoretical basis for breeding high quality C.oleifera varieties.

关 键 词：油茶 油脂代谢 不饱和脂肪酸 蛋白质组 差异表达蛋白 

分 类 号：S794.4[农业科学—林木遗传育种]