呼吸道合胞病毒非结构蛋白1氨基酸变异情况及临床特征分析  

Analysis of respiratory syncytial virus nonstructural protein 1 amino acid variation and clinical characteristics

作　　者：翟惠 高磊琼 任洛[1] 谢军[1] 刘恩梅[1] Zhai Hui;Gao Leiqiong;Ren Luo;Xie Jun;Liu Enmei(Department of Respiratory Medicine,Children′s Hospital of Chongqing Medical University,National Clinical Research Center for Child Health and Disorders,Ministry of Education Key Laboratory of Child Development and Disorders,Chongqing Key Laboratory of Child Infection and Immunity,Chongqing 400014,China)

机构地区：[1]重庆医科大学附属儿童医院呼吸科,国家儿童健康与疾病临床医学研究中心,儿童发育疾病研究教育部重点实验室,儿童感染免疫重庆市重点实验室,重庆400014

出　　处：《中华儿科杂志》2023年第8期695-699,共5页Chinese Journal of Pediatrics

基　　金：重庆市自然科学基金重点项目(cst2019jcyi-zdxmX0030)。

摘　　要：目的探讨呼吸道合胞病毒非结构蛋白(NS)1氨基酸变异情况及临床特征的关系。方法回顾性病例研究。纳入2018年12月至2020年1月重庆医科大学附属儿童医院呼吸科住院的81例单一呼吸道合胞病毒阳性患儿为研究对象,分别对呼吸道合胞病毒A、B亚型NS1基因进行PCR扩增、测序,对氨基酸序列进行分析。采用χ^(2)检验和Mann-Whitney秩和检验比较NS1有无变异两组患儿临床特征及其Ⅰ型干扰素水平。结果81例单一呼吸道合胞病毒阳性患儿中男58例、女23例。变异组11例,发病年龄2.0(1.0,11.0)月龄,A亚型4例,变异位点分别为Lys33Gln 2例,Gly2Asp、Pro67Ser、Leu137Phe各1例;B亚型7例,变异位点分别为Val121Ile 2例,Tyr30Cys、Val65Met、Asn85Ser、Ser118Asn、Asp124Asn各1例;所有变异位点在美国国家生物技术信息中心蛋白质数据库中的频率为0.08(0.04,0.29)%。无变异组70例,发病年龄为3.5(1.0,7.0)月龄。变异组呼吸困难发生率高于无变异组[10/11比47%(33/70),χ^(2)=7.31,P<0.01]。结论呼吸道合胞病毒NS1氨基酸存在一定的变异,变异后患儿可能更易出现呼吸困难。ObjectiveTo investigate the relationship between amino acid variations of respiratory syncytial virus(RSV)nonstructural protein(NS)1 and the clinical characteristics.MethodA retrospective case review was conducted.From December 2018 to January 2020,a total of 81 cases of hospitalized children who were tested only positive for RSV by RT-PCR or PCR at the Department of Respiratory Medicine,Children′s Hospital of Chongqing Medical University were included in the study.The NS1 genes of RSV subtype A and subtype B were amplified by PCR and sequenced.The amino acid sequences were analyzed.The Chi-square test and Mann-Whitney rank sum test were used to compare the clinical characteristics and typeⅠinterferon levels of children with or without NS1 variation in the variation and non-variation groups.ResultsAmong 81 cases,there were 58 males and 23 females.There were 11 cases in the variation group,the age of onset was 2.0(1.0,11.0)months,included 4 cases of subtype A(variant sites were:2 cases for Lys33Gln,one case for Gly2Asp,Pro67Ser,Leu137Phe,respectively)and 7 cases of subtype B(variant sites were:two cases for Val121Ile,one case for Tyr30Cys,Val65Met,Asn85Ser,Ser118Asn,Asp124Asn,respectively).These variant sites all appeared at a very low frequency 0.08(0.04,0.29)%in the NCBI PROTEIN database.There were 70 cases in non-variation group,the onset age was 3.5(1.0,7.0)months.The proportion of dyspnea in the variation group was higher than that in the non-variation group(10/11vs.47%(33/70),χ^(2)=7.31,P<0.01).ConclusionsThere are some variant sites in nonstructural protein NS1 of RSV.Children may be prone to have dyspnea with NS1 variations.

关 键 词：呼吸道合胞病毒 病毒非结构蛋白NS1 氨基酸变异 临床特征 干扰素Ⅰ型 

分 类 号：R725.6[医药卫生—儿科]