我国15种典型土壤中菲对白符跳的毒性阈值及其预测模型  

Toxicity Threshold and Prediction Model of Phenanthrene to Folsomia candida in 15 Typical Soils in China

作　　者：冯献捷 王冕 韩东锦 侯红[2] 赵龙[2] 左晓俊 FENG Xianjie;WANG Mian;HAN Dongjin;HOU Hong;ZHAO Long;ZUO Xiaojun(Nanjing University of information Science and Technology,Nanjing 210044,China;State Key Laboratory of Environmental Criteria and Risk Assessment,Chinese Research Academy of Environmental Sciences,Beijing 100012,China)

机构地区：[1]南京信息工程大学,江苏南京210044 [2]中国环境科学研究院,环境基准与风险评估国家重点实验室,北京100012

出　　处：《环境科学研究》2023年第8期1625-1633,共9页Research of Environmental Sciences

基　　金：国家重点研发计划项目(No.2019YFC1804603)。

摘　　要：为了确定我国土壤中菲(Phe)对白符跳(Folsomia candida)的毒性阈值并建立其预测模型,以外源添加的方式研究了我国15种典型土壤中Phe对白符跳存活率与繁殖率的影响.结果表明:(1)白符跳繁殖率对Phe毒害的敏感性远高于存活率,基于混合有机溶剂(正己烷与丙酮的体积比为1∶1)提取的Phe实测值推导的繁殖率的EC_(50)(半数效应浓度)范围为21.09~99.50 mg/kg,不同土壤中的EC_(50)最大值是最小值的4.72倍;基于HPCD(羟丙基-β-环糊精)提取的Phe实测值推导的繁殖率的EC_(50)范围为18.31~48.26 mg/kg,不同土壤中的EC_(50)最大值是最小值的2.63倍.(2)对白符跳繁殖的EC_(50)与土壤理化性质进行Pearson相关性分析表明,EC_(50)与土壤有机质含量、黏土颗粒占比均呈显著正相关,相关系数分别为0.923、0.656;与土壤pH呈显著负相关,相关系数为-0.590.(3)利用多元逐步回归分析方法对白符跳繁殖的EC_(50)及土壤理化性质进行分析,发现土壤有机质含量可以很好地解释不同土壤中白符跳繁殖的EC_(50)值之间的差异,故利用土壤有机质含量建立了白符跳繁殖的EC_(50)值预测模型—lg(EC_(50))=1.436+0.012[OM]([OM]表示土壤有机质含量).该毒性预测模型显示,土壤有机质含量是影响不同土壤中Phe对白符跳繁殖的EC_(50)毒性差异的最重要的单一因素,可解释不同土壤中EC_(50)值84.0%的差异.In order to determine the toxicity threshold of phenanthrene(Phe)to Folsomia canadida in Chinese soils and to establish a prediction model for Phe toxicity,we investigated the effects of exogenous Phe on the survival and reproduction of the model organism Folsomia candida in 15 typical soils.The results showed that the sensitivity of survival to Phe toxicity was much lower than that of reproduction.The EC_(50)(half effect concentration)of the reproduction rate derived from the Phe value of mixed organic solvent(1:1 volume mixture of n-hexane and acetone)extraction ranged from 21.09 mg/kg to 99.50 mg/kg with a difference of 4.72 times.The EC_(50) of the reproduction rate derived from the Phe value of mild HPCD(Hydroxypropyl-β-Cyclodextrin)extraction ranged from 18.31 mg/kg to 48.26 mg/kg with a difference of 2.63 times.Furthermore,pearson correlation analysis revealed that the EC_(50) values based on the Phe values of organic solvent extraction were significantly correlated with soil organic matter and soil clay,with the correlation coefficients of 0.923 and 0.656,respectively.The EC_(50) values were significantly negatively correlated with soil pH,with the correlation coefficients of−0.590.For the Phe toxicity thresholds for reproduction,we developed a prediction model lg(EC_(50))=1.436+0.012[OM]based on soil organic matter using multiple stepwise regression analysis.The toxicity prediction model shows that the content of Soil organic matter is the most important single factor affecting the difference in the toxicity of Phe to the EC_(50) in different soils,which can explain 84.0%of the difference in the EC_(50) value in different soils.

关 键 词：白符跳 菲(Phe) 土壤理化性质 外源添加 毒性预测模型 

分 类 号：X53[环境科学与工程—环境工程]