Genome-wide identification and characterization of the IPT family members in nine Rosaceae species and a functional analysis of MdIPT5b in cold resistance  

作　　者：Yi Feng Jiahong Lv Mengqun Peng Jie Li Yue Wu Min Gao Xinyi Wu Yi Wang Ting Wu Xinzhong Zhang Xuefeng Xu Zhenhai Han 

机构地区：[1]College of Horticulture,China Agricultural University,Beijing,100193,China [2]State Key Laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,China Agricultural University,Beijing,100193,China

出　　处：《Horticultural Plant Journal》2023年第4期616-630,共15页园艺学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant Nos. 32172522 and 31901974);the China Postdoctoral Science Foundation (Grant No. 2018M640205);the Earmarked Fund for China Agriculture Research System (Grant No. CARS-27);the 2115 Talent Development Program of China Agricultural University;the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Nutrition and Physiology) in the Ministry of Agriculture and Rural Affairs, China

摘　　要：Cytokinins are members of a group of phytohormones involved in various growth and developmental processes in plants.Isopentenyl transferase(IPT)is the rate-limiting enzyme in catalyzing the biosynthesis of cytokinins.In this study,to understand the role of IPT family in cold resistance,78 IPT candidates were identified and characterized in nine Rosaceae genomes.The expansion of IPT families in the Rosaceae primarily occurred through segmental duplication rather than tandem duplication.In general,purifying selection controlled the evolution of IPT families in the Rosaceae,with IPT3 and IPT5 homologs as the primary drivers of evolution.Cis-elements,which are involved in the responses to many environmental stresses or phytohormone signals,were identified in the promoters of MdIPT members.This was consistent with the trends of expression of the MdIPT genes in apple(Malus domestica)calli.MdIPT5b was also found to exhibit multiple responses to phytohormones and stress signals.The ectopic expression of MdIPT5b resulted in an increase in cold resistance in transformed apple calli and tomato(Solanum lycopersicum)plantlets.The redox balance was partially stabilized through the accumulation of proline under cold stress.However,the ascorbate–glutathione cycle cannot be stabilized in the cold.All physiological and biochemical assays are preformed in spectrophotometer.These results showed that regulating the expression of IPT genes for moderate cytokinin improvement could enhance the accumulation of proline to stabilize the osmotic and redox balances to improve resistance to cold stress.

关 键 词：ROSACEAE Apple Isopentenyl transferase CYTOKININ Cold stress Bioinformatics 

分 类 号：Q943.2[生物学—植物学]