Genome-wide identification of the mitogen-activated protein kinase kinases in pear and their functional analysis in response to black spot  

作　　者：Zan Zhang Qiming Chen Luting Jia Ming Qian Qinghai Qiao Xiaosan Huang Shaoling Zhang 

机构地区：[1]College of Horticulture,State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China

出　　处：《Horticultural Plant Journal》2023年第4期681-692,共12页园艺学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(Grant No.2022YFD1200503);Jiangsu Agriculture Science and Technology Innovation Fund[Grant Nos.SCX(22)3215],Fundamental Research Funds for the Central Universities(Grant No.JCQY201901);the Earmarked Fund for China Agriculture Research System(Grant No.CARS-28).

摘　　要：The mitogen-activated protein kinase(MAPK)cascade is crucial to plant growth,development,and stress responses.MAPK kinases(MAPKK)play a vital role in linking upstream MAPKK kinases(MAPKKK)with the downstream MAPK.Black spot is one of the most serious fungal diseases of pear which is an important part of the fruit industry in China.The MAPKK genes have been identified in many plants,however,none has been reported in pear(Pyrus bretschneideri).In order to explore whether MAPK gene of pear is related to black spot disease,we designed this experiment.The present study investigated eight putative PbrMAPKK genes obtained from the Chinese white pear genome.The phylogenetic analysis revealed that PbrMAPKK genes were divided into A,B,C,and D groups.These PbrMAPKK genes are randomly distributed on 7 out of 17 chromosomes and mainly originated from the whole-genome duplication(WGD)event.The expression analysis of PbrMAPKK genes in seven pear tissues and the leaves of susceptible and resistant varieties after Alternaria alternata infection by quantitative real-time PCR(qRT-PCR)identified seven candidate genes associated with resistance.Furthermore,virus-induced gene silencing(VIGS)indicated that PbrMAPKK6 gene enhanced resistance to pear black spot disease in pear.

关 键 词：Pyrus bretschneideri Mitogen-activated protein kinase kinase(MAPKK) Gene family Disease resistance Pear black spot 

分 类 号：S661.2[农业科学—果树学]