Comparative genomic analysis of N6-methyladenosine regulators in nine rosaceae species and functional characterization in response to drought stress in pear  

作　　者：Chenyang Han Huizhen Dong Qinghai Qiao Yuqin Dai Xiaosan Huang Shaoling Zhang 

机构地区：[1]College of Horticulture,State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China

出　　处：《Horticultural Plant Journal》2023年第4期693-704,共12页园艺学报（英文版）

基　　金：supported by the National Key Research and Development Program of China (Grant No. 2019YFD1000102);the National Science Foundation of China (Grant Nos. 31872070, 32072538);the Jiangsu Agriculture Science and Technology Innovation Fund (CX Grant No. 183065);the Fundamental Research Funds for the Central Universities of Nanjing Agricultural University (Grant No. KYZ201607);the College of Horticulture SRT project of the Nanjing Agriculture University (Grant No. 202011YX05);the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions;the Undergraduate Training Program for Innovation and Entrepreneurship (Grant No. S20190040)

摘　　要：N 6-methylated adenine(m6 A)is an emerging epigenetic marker in eukaryotic organisms that plays an important role in biological functions and in enriching genetic information.m6 A exerts these functions via the dynamic interplay among m6 A writers,erasers,and readers.However,little is known about the underlying mechanisms of m6 A in plant growth and stress responses.Here,we identified 276 masked m6 A regulators from nine Rosaceae species(Pyrus bretschneideri,Pyrus betulifolia,Pyrus communis,Malus domestica,Fragaria vesca,Prunus avium,Prunus mume,Prunus persica,and Rubus occidentalis).We classified and named these genes in more detail based on phylogenetic and synteny analysis.The expansion of m6 A regulators in Maloideae was dated back to the recent whole-genome duplication(WGD)in Rosaceae.Based on the expression pattern analysis and gene structure analysis of m6 A regulators,m6 A was shown to be a significant factor in regulating plant development and resistance.In addition,PbrMTA1-silenced pear plants displayed significantly reduced drought tolerance and chlorophyll content,as well as increased electrolyte leakage and concentrations of malondialdehyde and H2 O2.

关 键 词：Pyrus bretschneideri RNA methylation m6A regulators ROSACEAE Evolution Transcriptome Drought stress VIGS 

分 类 号：Q943.2[生物学—植物学]