Mapping of QTLs and candidate genes associated with multiple phenotypic traits for Huanglongbing tolerance in citrus  

作　　者：Ming Huang Mikeal L. Roose Qibin Yu Ed Stover David G. Hall Zhanao Deng Frederick G.Gmitter Jr. 

机构地区：[1]Key Laboratory of Plant Hormones and Development Regulation of Chongqing,College of Life Sciences,Chongqing University,Chongqing 401331,China [2]Citrus Research and Education Center,University of Florida,Lake Alfred,FL 33850,United States [3]Department of Botany and Plant Sciences,University of California,Riverside,CA 92521,United States [4]United States Horticultural Research Laboratory,Agricultural Research Service,United States Department of Agriculture,Fort Pierce,FL 34945,United States [5]Gulf Coast Research and Education Center,University of Florida,Wimauma,FL 33598,United States

出　　处：《Horticultural Plant Journal》2023年第4期705-719,共15页园艺学报（英文版）

基　　金：supported by grants from the Citrus Research and Development Foundation,USA(Grant No.CRDF#15-010);the New Varieties Development and Management Corporation(NVDMC),on behalf of the Florida citrus industry,USA,the Fundamental Research Funds for the Central Universities,China(Grant No.2022CDJXY-004);from the USDA-NIFA-SCRI,USA(Grant No.2015-70016-2302).

摘　　要：Huanglongbing(HLB)is the most devastating disease for citrus worldwide.Candidatus Liberibacter asiaticus(C Las),vectored by Asian citrus psyllid(ACP,Diaphorina citri Kuwayama),is the most common pathogen causing the disease.Commercial citrus varieties are highly susceptible to HLB,whereas trifoliate orange(Poncirus trifoliata)is considered highly tolerant to HLB.An F1 segregating population and their parent trifoliate orange and sweet orange,which had been exposed to intense HLB pressure for three years,was evaluated for disease symptoms,ACP colonization,C Las titer and tree vigor repeatedly for two to three years.Trifoliate orange and sweet orange showed significant differences for most of the phenotypic traits,and the F1 population exhibited a large variation.A high-density SNP-based genetic map with 1402 markers was constructed for trifoliate orange,which exhibited high synteny and high coverage of its reference genome.A total of 26 quantitative trait locus(QTLs)were identified in four linkage groups LG-t6,LG-t7,LG-t8 and LG-t9,of which four QTL clusters exhibit a clear co-localization of QTLs associated with different traits.Through genome-wide analysis of gene expression in response to C Las infection in‘Flying Dragon’and‘Larger-Flower DPI-50-7’trifoliate orange,85 differentially expressed genes were found located within the QTL clusters.Among them,seven genes were classified as defense or immunity protein which exhibited the highest transcriptional change after C Las infection.Our results indicate a quantitative genetic nature of HLB tolerance and identified candidate genes that should be valuable for searching for genetic solutions to HLB through breeding or genetic engineering.

关 键 词：Candidatus liberibacter QTL mapping PHENOTYPING F1 population PONCIRUS CITRUS 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]