Mining candidate genes of grape berry cracking based on high density genetic map  

作　　者：Chuan Zhang Liwen Cui Chonghuai Liu Xiucai Fan Jinggui Fang 

机构地区：[1]Institute of Grape Science and Engineering,College of Horticulture,Qingdao Agricultural University,Qingdao,Shandong 266109,China [2]Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou,Henan 450009,China [3]College of Agro-grassland Science,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China

出　　处：《Horticultural Plant Journal》2023年第4期743-753,共11页园艺学报（英文版）

基　　金：financial support from the Highlevel Scientific Reuter Foundation of Qingdao Agricultural University(Grant Nos.665/1118011,665/1119002);China Agriculture Research System of MOF and MARA(Grant No.CARS-29-yc-1);Crop Resources Protection Program of Ministry of Agriculture and Rural Affairs of China(Grant No.2130135-34).

摘　　要：Fruit cracking is a phenomenon in which the peel cracks during grape berry development,which seriously affects the yield and quality of the fruit.However,there are few studies on the mining of candidate genes related to berry cracking.In order to better understand the genetic basis of berry cracking,we used the results of previous quantitative trait locus(QTL)mapping,combined with field surveys of berry-cracking types and the berry-cracking rate,to mine candidate berry-cracking genes.The results showed that three identical QTL loci were detected in two years(2019 and 2020);and three candidate genes were annotated in the QTL interval.In mature berries,the expressions of the candidate genes were more abundant in the cracking-susceptible parent(‘Crimson Seedless’)than in the cracking-resistant parent(‘Muscat Hamburg’).Grape berry cracking is a complex trait controlled by multiple genes,mainly including genes encoding cellulose synthase–like protein H1,glucan endo-1,3-beta-glucosidase 12,and brassinosteroid insensitive 1-associated receptor kinase 1.The high expression of the candidate berry-cracking genes may promote the occurrence of berry cracking.This study helps elucidate the genetic mechanism of grape berry cracking.

关 键 词：Grape berry Berry-cracking rate Berry-cracking type Candidate gene Quantitative trait loci 

分 类 号：S663.1[农业科学—果树学]